SKU: 79804063612

mrp1 vesicle kit

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Description

mrp1 vesicle kitHuman MRP1 Vesikel Kit Inside Out Membranvesikel fr Multidrugresistenz und organische Anionen Transportstudien Das Human MRP1 Vesikel Kit (HEK293 abgeleitet) liefert gebrauchsfertige Inside Out Membranvesikel fr ATP abhngige Transportstudien des Multidrug Resistenz assoziierten Proteins 1 (MRP1 ABCC1). Jedes Kit enthlt Material fr 100 Reaktionen mit einem garantierten ATP AMP Verhltnis von >5. MRP1 vermittelt den Efflux von Glutathion , Glucuronid und

Human MRP1 Vesikel-Kit – Inside-Out-Membranvesikel für Multidrugresistenz- und organische Anionen-Transportstudien

Das Human MRP1 Vesikel-Kit (HEK293-abgeleitet) liefert gebrauchsfertige Inside-Out-Membranvesikel für ATP-abhängige Transportstudien des Multidrug-Resistenz-assoziierten Proteins 1 (MRP1 / ABCC1). Jedes Kit enthält Material für 100 Reaktionen mit einem garantierten ATP/AMP-Verhältnis von >5. MRP1 vermittelt den Efflux von Glutathion-, Glucuronid- und Sulfatkonjugaten sowie einer breiten Palette organischer Anionen einschließlich Krebsmittel — was es zu einem wichtigen Transporter in onkologischen DDI-Studien und Gewebeverteilungsprofilen macht.

Transporterprofil

MRP1 (ABCC1) wird breit in Lunge, Hoden, Niere, Blut-Hirn-Schranke, Herz- und Skelettmuskel, Plazenta und Makrophagen exprimiert. Es transportiert Glutathion (GSH), Glutathionkonjugate (GS-X), Glucuronide, Sulfatkonjugate und lipophile Arzneimittel in Komplex mit Glutathion. Wichtige Substrate: E217βG (Estradiol-17β-Glucuronid), GSSG, Saquinavir, Ritonavir, Methotrexat, Daunorubicin, Leukotriene. Bekannte Hemmer: MK-571, Probenecid, Indometacin. MRP1 ist keine primäre regulatorische Anforderung, ist aber relevant für Verbindungen mit signifikanter Gewebeverteilung oder onkologischen Anwendungen.

Technische Spezifikationen

ParameterSpezifikation
TransporterHuman MRP1 (Multidrug Resistance-Associated Protein 1) (ABCC1)
ExpressionssystemHEK293 (transiente Überexpression)
VesikeltypInside-Out-Membranvesikel
Reaktionen pro Kit100
ATP/AMP-Transportverhältnis>5
DetektionskompatibilitätRadiolabel (³H-E217βG), LC-MS/MS, fluorescence
Lagerung−80 °C (Lieferung auf Trockeneis)
Kit-InhaltVesikel, ATP, AMP, Reaktionspuffer, Waschpuffer, CoA
HerstellerCell4Pharma (Netherlands)
Distributor DACHSeamlessBio GmbH, Passau

Anwendungsgebiete

AnwendungBeschreibung
Multidrugresistenz (MDR)-ProfilingBewertung ob Krebsmittel MRP1-Substrate sind — relevant für Tumor-Arzneimittel-Efflux, Resistenzmechanismen und kombinierte Therapieplanung.
Glutathion/Glucuronid-Konjugat-TransportCharakterisierung des Phase-II-Metaboliten-Efflux über MRP1 — wichtig für das Verständnis der systemischen Exposition konjugierter Arzneimittelmetaboliten.
Blut-Hirn-Schranke VerteilungMRP1-Expression an der BHS trägt zur eingeschränkten ZNS-Arzneimittelzugänglichkeit bei. Relevant für ZNS-Wirkstoffkandidaten, bei denen die Gehirndurchdringung charakterisiert werden muss.
Gewebs-DDI-ProfilingBewertung MRP1-vermittelter Arzneimittelinteraktionen an Expressionsorten — Lungen-, Herz- und Immunzellmodelle.

Assay-Prinzip

Inside-Out-Membranvesikel präsentieren die zytoplasmatische Seite des Transporters dem Assaypuffer und ermöglichen so ATP-abhängige Substrataufnahme in das Vesikelinnere. Vesikel mit Testverbindung und Probesubstrat in Gegenwart von ATP (Transport) oder AMP (Hintergrundkontrolle) inkubieren. Reaktion mit kaltem Waschpuffer stoppen, Vesikel durch Filtration trennen und akkumuliertes Substrat per LC-MS/MS, Radiolabel oder Fluoreszenz quantifizieren. Das ATP/AMP-Verhältnis bestimmt das Assay-Fenster für die zuverlässige IC₅₀-Bestimmung.

Komplettes Cell4Pharma Kit-Portfolio bei SeamlessBio

Alle ABC-Transporter-Vesikel-Kits aus einer europäischen Quelle — schnelle Kühlkettenlieferung, garantierte ATP/AMP-Verhältnisse, vollständige Dokumentation.

KitTransporterGenATP/AMP-Verhältnis
Human BCRP Vesicle KitBCRPABCG2>10
Human BSEP Vesicle KitBSEPABCB11>10
Human MRP1 Vesicle KitMRP1ABCC1>5
Human MRP2 Vesicle KitMRP2ABCC2>20
Human MRP3 Vesicle KitMRP3ABCC3>5
Human MRP4 Vesicle KitMRP4ABCC4>5
Human MRP5 Vesicle KitMRP5ABCC5>5
Human MRP8 Vesicle KitMRP8ABCC11>5
Human Pgp Vesicle KitP-gp / MDR1ABCB1>5
Human Pgp + FluoPgp KitP-gp / MDR1ABCB1>5
Human Control Vesicle KitHEK293 empty vector

Qualität & Regulatorische Konformität

  • Produziert von Cell4Pharma — Spezialhersteller für ABC-Transporter-Vesikelsysteme
  • Garantiertes ATP/AMP-Transportverhältnis >5 pro Charge
  • Große, einheitliche Chargen — maximale Lot-zu-Lot-Konsistenz
  • Chargenspezifischer QC-Bericht + Datenblatt bei jedem Kit inklusive
  • Konform mit FDA DDI Guidance (2020) und EMA DDI Guideline (2013/2022)

Warum über SeamlessBio bestellen?

VorteilBeschreibung
✅ EU-LagerverfügbarkeitSchnelle Kühlkettenlieferung in Deutschland, Österreich, Schweiz und der EU
✅ DirektkontaktDirekter Kontakt zum Fachberater — auf Deutsch oder Englisch
✅ Vollständige DokumentationDatenblatt, QC-Bericht und Rechnung bei jeder Lieferung
✅ ChargenreservierungSpezifische Charge für die gesamte Projektlaufzeit reservieren
✅ Komplettes PortfolioAlle 11 Cell4Pharma Kits aus einer Quelle — BCRP, BSEP, MRP1–8, Pgp, Kontrolle

Bestellung oder Preisanfrage: [email protected] | +49 851 37932226

Häufige Fragen

Ist MRP1 eine regulatorische Anforderung für DDI-Einreichungen?

MRP1 ist nicht als obligatorischer Tier-1-Transporter von FDA oder EMA gelistet, wird aber für Verbindungen in onkologischen Indikationen, Verbindungen mit signifikanter Lungen-/Gehirn-/Herzverteilung oder solchen empfohlen, die extensive Phase-II-Metaboliten sind.

Welches Probesubstrat wird für MRP1 empfohlen?

E217βG (Estradiol-17β-D-Glucuronid) ist das Standardprobesubstrat für MRP1-Vesikelassays — erhältlich als ³H-markiert oder unmarkiert für LC-MS/MS. GSSG wird ebenfalls häufig verwendet.

Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
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Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 79804063612

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4.0 ★★★★★
Based on 21 reviews
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MrDerp
Los Angeles, US
★★★★★ 3
Not an average-to-large person's chair by any means.
Color: Brown, Number of Items: 1
PROS: Comfortable seat cushion Feels sturdy and manufactured well Swivels and rolls effortlessly Height adjustment Not terribly heavy Easy assembly CONS: Reclining feature is not great Back cushion forces a very upright sitting posture Pleather material will deteriorate within a couple years It's overpriced for what it is, even at $100. SUMMARY: I'm a little guy. 5'7 and 145 lbs. This chair feels like it was scaled for me and my little frame. Now, while that's perfect for me, nearly everyone else I know or have ever known, are at least twice my size. And I can't imagine them finding the overall size of this thing to be enough chair for them. Maybe I'm way off base here, but I've sat in regular sized office chairs and while they'd swallow me, they're essentially perfect sized for most other people. Feels good to sit in, nothing amazing but it's comfortable enough. Remember, it's made with pleather. Every day use for long periods of time will wear the material out within two or three years. Won't effect it's performance but it will be ugly with cracks and wear/tear far quicker than real leather, of course. It looks very nice right out the box though. Not too heavy either. The reclining feature is a bummer, it's like you really have to force it to lean back, like it's fighting against your doing so. Which if your trying to lean back, you know putting a bunch of effort into your legs is the opposite of what you're trying to achieve - which is relax without tension in any part of your body. It's basically useless for my needs, anyway. The price, even at a measly $100 for an "executive office chair" does not reflect the hiccups with this thing because it's not a one size fits all kind of chair. Anyone over 180 lbs and 5'10 will surely find it to be a bit snug and the back cushion to sit a bit short. The back forces you to sit very upright. Something that will take getting used to, especially since I can't recline. This chair is kinda "meh" to be completely honest. I'll spend at least twice as much next time and get five times the quality. Look, it'll do the job for a while but there really isn't anything very impressive about it besides what it visually looks like. I've no idea how so many people rated this thing with so many glowing reviews. There's not a lot to boast about with a cheap $100 pleather chair that's undersized for most people. I really don't get it. I'd only recommend this at $65-$70. No way this is worth the $100 Amazon is currently pricing it at. Spend just a little a bit more, you'll notice a huge jump in quality over this.
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Reviewed in the United States on August 15, 2024
M
Verified Purchase
MrTruth
Battle Creek, US
★★★★★ 3
Just Great!
Color: Beige, Number of Items: 1
Comfortable. Wheels roll very well. Great padding. Seems durable. Assembly is the standard with such chairs, takes about 15 minuttes. Do not know how long the PL? leather fabric will last? Probably wear in a couple of years. But for the price, this is perfect. UPDATE: After 2 1/2 months, seat feels flat and very uncomfortable.
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Reviewed in the United States on March 12, 2026
V
Verified Purchase
VicYay
Omaha, US
★★★★★ 5
Modern and comfortable, easy to assemble
Color: White, Color: White
Reliable chair with solid quality. Assembly took about 35 mins following the clear instruction sheet. Chair can hold at least 220 lbs, stable and easy to move. Wheels can move in multiple directions. Seat heights and arm are both adjustable. No stain or scratches on the padding surface. White finish looks clean and modern.
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Reviewed in the United States on April 27, 2026
B
Verified Purchase
boo h 1964
Phoenix, US
★★★★★ 5
Impressive Chair for the price!
Color: Black
Purchased for my mother, she absolutely loves it. She used a secretary style chair forever and said she didn't want a big bulky chair like the executive type until she saw this one. It's comfortable like an executive chair but not quite a big or cumbersome as the executive can be. It took a hot minute to put together but well worth it. Instructions were very clear and easy, I'm in my 60's, if I can put it together anybody can! Chair is well made, solid!
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Reviewed in the United States on May 8, 2026
R
Verified Purchase
Ronnie
Bozeman, US
★★★★★ 4
Above average
Color: Black
Easy to assemble. Fairly comfortable for the price. although I thought that the seat cushion would be more soft. overall I'm satisfied.
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Reviewed in the United States on June 5, 2026

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