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Description
pgp fluopgp vesicle kitDas Human BCRP Vesicle Kit (HEK293 derived) liefert einsatzfertige Inside out Membranvesikel fr ATP abhngige Transportstudien mit dem Breast Cancer Resistance Protein (BCRP ABCG2). Hergestellt von Cell4Pharma aus transient transfizierten HEK293 Zellen, enthlt jedes Kit Material fr 100 Reaktionen und wird mit einem garantierten ATP AMP Transportverhltnis von >10 geliefert assay ready direkt aus dem Tiefkhlschrank. BCRP (ABCG2) ist einer der klinisch
Das Human BCRP Vesicle Kit (HEK293 derived) liefert einsatzfertige Inside-out-Membranvesikel für ATP-abhängige Transportstudien mit dem Breast Cancer Resistance Protein (BCRP / ABCG2). Hergestellt von Cell4Pharma aus transient transfizierten HEK293-Zellen, enthält jedes Kit Material für 100 Reaktionen und wird mit einem garantierten ATP/AMP-Transportverhältnis von >10 geliefert – assay-ready direkt aus dem Tiefkühlschrank.
BCRP (ABCG2) ist einer der klinisch bedeutsamsten Efflux-Transporter und wird in den Leitlinien der FDA (2020) und EMA (2013) explizit als Pflichtuntersuchung für Drug-Drug-Interaction-Studien (DDI) benannt. Ob Sie Ihre neue chemische Entität als BCRP-Substrat oder -Inhibitor charakterisieren möchten – dieses Kit bietet die validierte, humane biologische Plattform, die Sie für Ihre regulatorischen Studien benötigen.
Transporterprofil
| Genname | ABCG2 |
| Synonyme | BCRP, MXR, ABCP, CD338 |
| Proteinfamilie | ATP-Binding Cassette (ABC) Transporter, Subfamilie G |
| Gewebeexpression | Plazenta, Dünndarm, Leber, Gehirn (Blut-Hirn-Schranke), Niere, Brustdrüse, Stammzellen |
| Repräsentative Substrate | Estron-3-sulfat (E1S), Rosuvastatin, Methotrexat, Irinotecan, Topotecan, Sulfasalazin, Pantoprazol, Chlorothiazid |
| Referenzinhibitoren | Ko143, Fumitremorgin C (FTC), Elacridar (GF120918) |
| Regulatorische Relevanz | Explizit gefordert durch FDA DDI Guidance (2020) und EMA DDI-Leitlinie (2013) |
Kit-Inhalt (100 Reaktionen)
- BCRP-Membranvesikel (HEK293-deriviert) — ATP/AMP-Verhältnis >10
- Kontrollmembranvesikel (HEK293, Leervektor-deriviert)
- ATP-Lösung (Reaktionsqualität)
- AMP-Lösung (Reaktionsqualität)
- Assay-Puffer (optimiert für vesikulären Transport)
- Stopplösung / Waschpuffer
- Produktdatenblatt inkl. chargenspezifischer QC-Daten
Die Detektion des transportierten Substrats ist per Radiomarkierung, Fluoreszenz oder LC-MS/MS möglich. Substrat und Radiomarkierung sind nicht im Kit enthalten und müssen separat bezogen werden. Versand auf Trockeneis; bei −80 °C lagern.
Assay-Prinzip
Humanes BCRP wird transient in HEK293-Zellen überexprimiert und als Inside-out-Membranvesikel isoliert. In dieser Orientierung zeigt die substratbindende Seite von BCRP nach außen, wodurch die direkte Messung der ATP-abhängigen Substrataufnahme in das Vesikelinnere möglich ist. Die Inkubation einer Testverbindung mit den Vesikeln in Gegenwart von ATP (aktiver Transport) bzw. AMP (Kontrolle) erlaubt die Bestimmung, ob die Verbindung ein BCRP-Substrat oder -Inhibitor ist. Der Assay ist besonders geeignet für Verbindungen mit niedriger bis moderater passiver Permeabilität, bei denen Zell-Monolayer-Assays durch unspezifische Permeation verfälscht werden könnten.
Anwendungen
- Substratidentifizierung: Bestimmung, ob eine neue chemische Entität (NCE) ein BCRP-Substrat ist – regulatorische Pflichtuntersuchung für IND/NDA-Einreichungen.
- Inhibition / IC₅₀-Bestimmung: Quantifizierung der inhibitorischen Potenz von Testverbindungen gegenüber dem BCRP-vermittelten Transport eines Probe-Substrats.
- DDI-Risikoabschätzung: Erfüllung der EMA- und FDA-Anforderungen für transporter-basierte DDI-Bewertung im präklinischen Drug-Development-Paket.
- Onkologie-Wirkstoffentwicklung: Bewertung BCRP-vermittelter Multiresistenzmechanismen für Onkologie-Verbindungen.
- Bioverfügbarkeit / Absorptionsstudien: Untersuchung intestinaler BCRP-Efflux-Hemmung der oralen Bioverfügbarkeit.
- ZNS-Penetrationsstudien: Analyse des BCRP-Beitrags zur ZNS-Ausgrenzung an der Blut-Hirn-Schranke.
Qualität & Regulatorische Konformität
- Hergestellt von Cell4Pharma – Spezialhersteller humaner ABC-Transporter-Vesikelsysteme
- Garantiertes ATP/AMP-Transportverhältnis >10 pro Charge
- Große, homogene Chargen mit Lot-zu-Lot-Konsistenz
- Vollständige Produktdokumentation: Datenblatt und chargenspezifischer QC-Report im Lieferumfang
- Konform mit FDA DDI Guidance (2020) und EMA DDI-Leitlinie (2013/2022) für In-vitro-Transporterstudien
- Kompatibel mit Radiomarkierung, Fluoreszenz und LC-MS/MS-Detektion
Warum über SeamlessBio bestellen?
- ✅ EU-Lagerbestand – schnelle Kaltkettenlieferung in DACH und EU
- ✅ Direkter Kontakt – Sie sprechen mit einem Wissenschaftler, nicht mit einem Callcenter
- ✅ Vollständige Dokumentation – Datenblatt, CoA und Rechnung bei jeder Lieferung
- ✅ Flexible Abnahmemengen – Einzelkit oder Projektversorgung mit mehreren Kits
- ✅ Chargenreservierung – Reservierung einer spezifischen Lot-Nummer für die gesamte Projektlaufzeit möglich
- ✅ Komplettes Cell4Pharma-Portfolio – BSEP, Pgp, MRP1–5, MRP8 und Kontrollvesikel-Kits aus einer Hand
Technische Spezifikationen
| Transporter | Humanes BCRP (ABCG2) |
| Expressionssystem | HEK293 (transiente Überexpression) |
| Vesikeltyp | Inside-out Membranvesikel |
| Qualitätsindikator | ATP/AMP-Verhältnis >10 (chargengarantiert) |
| Kit-Größe | 100 Reaktionen |
| Kompatible Detektion | Radiomarkierung, Fluoreszenz, LC-MS/MS |
| Lagerung | −80 °C |
| Versand | Trockeneis (Kaltkettenversand) |
| Hersteller | Cell4Pharma (Vertrieb durch SeamlessBio) |
| Regulatorische Ausrichtung | FDA DDI Guidance 2020, EMA DDI-Leitlinie 2013/2022 |
Preis und Verfügbarkeit auf Anfrage. Kontaktieren Sie uns direkt für ein Angebot, chargenspezifische Daten oder zur Projektversorgung und Chargenreservierung.
📞 +49 851 37932226 | 📧 [email protected] | 🌐 www.seamlessbio.de
📞 +49 851 37932226 | 📧 [email protected] | 🌐 www.seamlessbio.de
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
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4.7 ★★★★★
Based on 26 reviews
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Product Reviews
★★★★★ 5
Liked so much, I bought TWICE!
Color: 23 - Ice Blue, Size: Twin
These sheets are such a great quality for the price. They feel soft and comfortable right out of the package and make the bed feel so much more cozy. I’ve washed them multiple times and they still feel great with no fading or pilling.
The fit is also excellent — the fitted sheet stays securely in place and doesn’t slide off the corners during the night. I was so happy with the comfort and overall quality that I ended up purchasing a second set. Definitely worth the money and I would highly recommend them to anyone looking for comfortable, well-fitting sheets at a great price.
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Reviewed in the United States on May 14, 2026
★★★★★ 5
Finally we BOTH can sleep comfy!
Color: 08 - Black, Size: Queen
So for years my boyfriend has always liked “jersey sheets” or “tee shirt” sheets or sheets made of flannel. He says he doesn’t like the feeling of cold “silky” feeling sheets as he’s always cold. I’m opposite and am always hot, and anytime we stayed in a nice hotel I looked forward to being able to sleep on cooler, taught, soft sheets for a couple of nights. I was able to deal with our old sheets but the issue we both hated was after awhile any sheet we got would get pilling. We even splurged and got a $60 flannel sheet which did the same thing forcing me to shave off these little annoying fuzz balls that make it feels like there is thousands of crumbs on the sheets. I also would wash our bedding once a week because the Jersey sheets seemed to “grow” as we move around in bed making it so the sheet was not taught and super annoying to lay on. The day I would wash them they would be comfortable but ultimately by the end of the week they would be almost hanging off our mattress. I ordered these sheets on a whim and got them the same day and immediately just threw out the jersey sheet and put this on (I didn’t even wash it, I know it’s gross but I was desperate for a comfy nights sleep and they came around 8pm) when I put them on I realized how soft and comfortable they felt. I also liked how the pillow cases are larger than most pillow cases that I have had in the past. We’ve been using these sheets for about a week and I love how I don’t wake up with my pillow popping out of the case anymore. The sheets have loosed ever so slightly since a week of sleeping but still are really taught and comfortable. They are cooler than flannel or fabric sheets and are initially cold when you first lay on them but I wouldn’t consider them “cooling” but they keep me from feeing extremely hot. They are way better than I ever would have expected for the price. I look forward to laying on them at the end of each day, even my boyfriend laid in bed the day I got them and said “wow I didn’t think I would like sheets like this, these are so soft!” And fell asleep immediately. We ended up ordering a second set in grey and the color is pretty. I still have yet to wash them, I usually use 2 tide pods when washing the bedding but I might get some fabric softener (as I hear it helps with pilling) to prevent the possibility of having that happen to these sheets in the future. I am hoping they hold up well after the wash and longer use. If they start to pill after wash, I will come back and update my review. Until then, I would highly recommend these sheets for the quality and the price point that they are offered at. Honestly, at this point even if they pill months from now I probably wouldn’t even be upset as long as they stay the same price I would just buy another set as they are just that comfortable and soft.
UPDATE: So it has been about 3 months since I have purchase and used these sheets. They are still as soft as the day I first put them on. I’ve been washing them bi-weekly along with my comforter and pillowcases. I wash them with warm water using a combination of tide pods and added Downy fabric softener. They say to hang dry them in the description but I have been putting them in the dryer on medium and have been taking them out after around 20-25 minutes, they seem to dry extremely quickly. They have yet to have absolutely any pilling and also have not shrunk or stretched. Also I have a cat that is very active and also sleeps with me who enjoys kneading on my bed and I haven’t even noticed any signs of wear what so ever to the eye or to the feel. I highly recommend these sheets. I still have yet to even open the second set I purchased!
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Reviewed in the United States on January 10, 2024
★★★★★ 5
Amazing quality for the price! So fresh and lightweight
Color: 03 - Light Grey, Size: Queen
I am very impressed with this queen sheet set! I originally bought it because of the great price, but the quality has exceeded my expectations. The fabric is incredibly soft, lightweight, and stays very fresh throughout the night. It truly gives you that comfortable hotel bedding feel without breaking the bank. If you are looking for breathable and budget-friendly sheets, I highly recommend this set
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Reviewed in the United States on May 19, 2026
★★★★★ 4
Utopia bedding is GREAT
Color: 15 - Sage Green, Size: King
I had never heard of Utopia before I suddenly needed to buy bedding for my adult autistic son. He has sensory issues so shopping for anything that touches his body is a bit of a nightmare. I took a gamble on these sheets but it paid off big time! He says they are the most comfortable sheets he has ever slept on and that he doesn't overheat sleeping in them. What I liked about them is that the pockets were deep enough that it fit on his oversized plush mattress easily. I love the color and the ones I bought were very true to the picture. These are soft, comfy, they look nice and they are affordable. I liked this brand so much that I went ahead and got pillows and a comforter from them as well. Its is great for my son and I am very pleased to have found a fabric he tolerates.
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Reviewed in the United States on May 16, 2026
★★★★★ 5
For soft, sensually awesome sheets- LOOK no more!
Color: 15 - Sage Green, Size: Full
Would give 10 stars if possible! (And this is a nonpaid for review!) When I received the sheets, instantly I knew that I had the right ones, had bought a down comforter close in color as well.. these sheets? The material is strong yet, soft and warm. So so especially soft, and when I make up the bed, ahhh! plus the corners on the fitted, such a cinch to pull over the mattress, even with a topper on the bed! Feels almost spa-like <3 Very comfy, so far in the winter and in 85 degree heat! Soft and easy peasy to care for, too. Washing is a breeze, dry and fold. So far the only thing I can smell on these sheets is my wonderful detergent! Will purchase again. And possibly, again! Thank you!!
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Reviewed in the United States on May 21, 2026