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Description
Human TAZ ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a tafazzin (TAZ) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of tafazzin (TAZ) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Tafazzin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Tafazzin is a protein encoded by the TAFAZZIN gene. Tafazzin is highly expressed in cardiac and skeletal muscle and functions as a phospholipid-glycophospholipid transacylase (a member of the phospholipid:diacylglycerol acyltransferase family). It catalyzes the remodeling of immature cardiolipin into mature components, primarily tetrahydrooleoyl groups. The TAFAZZIN gene produces several distinct tafazzin protein isoforms. Each isoform has a long form and a short form. The short form lacks a hydrophobic leader sequence and may be a cytoplasmic protein rather than a membrane-bound protein. Other alternatively spliced transcripts have been described. Most isoforms are found in all tissues, but some are only found in certain cell types. The TAFAZZIN gene is located at position 28 on the q arm of chromosome X and spans 10,208 base pairs. It produces a 21.3 kDa protein composed of 184 amino acids. Mutations in the TAFAZZIN gene have been associated with mitochondrial defects, Bartter syndrome, dilated cardiomyopathy (DCM), hypertrophic DCM, endocardial fibrosis, left ventricular noncompression (LVNC), breast cancer, papillary thyroid carcinoma, non-small cell lung cancer, glioma, gastric cancer, thyroid tumors, and colorectal cancer. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.4 ★★★★★
Based on 13 reviews
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Product Reviews
★★★★★ 5
Comfortable and good quality
Color: Black
This is a great chair value. It’s easy to assemble, height adjustable, and comfortable to sit on. It has a good seat cushion and firm back support. I really recommend this product. And I just ordered another one for my wife’s sewing table because she tried to steal mine.
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Reviewed in the United States on May 25, 2026
★★★★★ 5
Inexpensive and decent, without any negative qualities.
Color: Black
Easy assembly with spare parts and the one important tool: the allen wrench that tightens those bolts. The instructions were clear and the parts well marked in the blister-package that is sturdy enough that cutting through the edges of the clear plastic bubbles to get at the parts with scissors is the best method to avoid having to chase the small parts when they fly out of the ruptured package. This chair uses the "New" kind of roller which is larger than the old ones and I was able to replace the standard tandem wheels with the super-slick roller blade type that are much faster and easier to maneuver. I'm sitting in the chair now as I type and it's very comfortable, even if the back isn't adjustable. The mesh back is much better for the Summer here (where it gets really hot!) and is more flexible than the basic upholstered chair back, so it fits and conforms. The one it replaced had been reworked several times and it was still too small with the back not correctly aligned, it had fallen apart anyway and was easier to throw away in small pieces.
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Reviewed in the United States on November 6, 2025
★★★★★ 4
Better Than Expected
Color: Black, Color: Black
Initial impressions: The chair is better quality than I expected. The seat material is nice: feels firm but soft; better than those seats with faux leather. So far, it is comfortable even for being over 200 lb and over 6 feet tall.
Many parts are sturdy metal, which is much better than plastic. The base that holds the wheels may be a form of plastic, but it is dense and feels strong; almost like metal.
The chair frame appears to be a firm plastic too, but not thin, so my initial thought is it will last. It has a buffed texture [not shiny or smooth -- which to me is nice, because it makes it look and feel more solid and not cheap.
The one weak point in design, in my opinion, is the cover that fits over the raising post: it feels a bit flimsy. Regardless, it probably wouldn't be noticeable to most unless they get down on the floor and squeeze it. Nevertheless, that there is a cover that raises and lowers like a telescope-- to "hide" the post, is a nice added attribute. A customer could leave the that cover off if they prefer, but I like it.
I appreciate that the casters stay in place (unlike the other chair I tested: the KK Toner Round Rolling Stool, whose wheels do not stay in place). This makes it possible to lift the chair without fear of dropping casters.
The casters are replaceable too, which is good. I was able to use Lifelong brand casters, instead, which are purportedly safe for hardwood and marble floors.
The seat is stationary once attached. It is mostly upright [not leaning back]. Even with its mesh backing it is firm. This is not a plush, rocking chair type of product, yet it is comfortable.
Instructions: As seems to be the new trend, they were mostly pictures without words. Regardless, putting the chair together was quite easy. Just make sure you doube check the front versus back of the seat -- this is important for setting the metal lift connector in place. And, don't forget to put the holding ring and washer on the screws.
They add an extra screw for the connector, and an extra screw, washer, and pressure ring for the back attachment. That was a pleasant surprise; and is appreciated.
The raising and lowering mechanism works well. You can sit on the chair while lowering it; it seems best to get off the chair while raiding it.
It lowers to where the seat is 28 inches off the floor. It rises to 22.5".
The seat is 17.5" wide x 16.25" front to back [the assembled chair is 18.5" front to back]. The back rest top to the floor is 34". The wheel base is about 20" at its widest point, before adding the wheel casters. With their standard wheels the width is about 21" to 21.5". With the Lifelong brand wheels, it will have a width of 21" to 22.5", depending on how the rotating wheel base is turned.
All in all, a good experience.
As I wrote, these are my initial impressions [I've only had the chair one day]. My intention is to use it daily as a dining chair. I will add to this review as time goes by [e.g., in ~ six months], to indicate how well this product does long-term and with moderately heavy use. I'd appreciate seeing more former reviewers do the same.
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Reviewed in the United States on January 10, 2023
★★★★★ 5
Rock bottom price, better than rock bottom quality
Color: Black
tl;dr there are definitely better chairs available, even better chairs that aren't that much more money, but if rock bottom is your price target, this one is hard to beat.
I bought my 7-year-old a $30 task chair at Target even knowing it's weight limit of 150 pounds implied it was of very, uh, questionable construction. He weighs 50 pounds though so I figured it'd be fine for occasional use for him. It was basically trash, however.
I found this instead and it's pretty solid for $60 delivered. The instructions are clear and easy to follow. The actual assembly is as easy as it gets for a task chair (snap casters in, put in a couple screws). Be aware that the seat base is 100% plastic, although I'd be astounded if anyone used cast aluminum for a seat base anywhere close to $100. It is not _particularly_ comfortable, ergonomic, or well made, although it is of slightly better construction than I was expecting. I'm 5' 11" and 200 pounds and the word I'd use to describe sitting on it is "acceptable". I suspect the seat foam is easily the weakest link regarding longevity if used by an adult.
The real benefit here is that $60 _feels_ like the correct price for a chair of this quality, whereas a local big box store is going to want close to double that, especially without a "sale" going on.
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Reviewed in the United States on January 31, 2026
★★★★★ 5
Easy to assemble, and comfortable!
Color: Blue
I bought this chair for my teenaged son, who has a small student's desk in his room (also ordered and assembled at the same time). This fits perfectly for the small desk, and is reasonably comfortable for a medium-sized adult, teenager, or older child. Once put together, the chair itself appears to work flawlessly, seems comfortable enough, the height is easily adjustable, and of course -- the most important part, according to my son -- "spins silently 'round and 'round really fast for a long time." The blue color is a nice, deep blue hue that works well for him and makes it stand out a little more than your average black chair. I was able to assemble it fairly quickly without issues. My son uses it to sit in for his schoolwork and computer gaming/programming/hobbies at his desk, etc. and he says it's comfortable, even after sitting in it for 1-2 hours at a time.
With this type of chair, you're getting exactly what you pay for and what you see in the pictures. No more, no less. The price is fairly reasonable, it feels sturdy, and if you're looking for an easy and simple office chair for light task work at a computer desk for a teenager or college student with limited space, this is an easy win. While it's not the smallest office chair out there, it's certainly not a large one, either. I am a medium-sized woman at 5'4", and my bottom doesn't hang off of it; that said, a taller/bigger person might want to look into something a little larger for comfort, if they have the space for it. My son is 5'6" (and growing) and slim, so it works perfectly well for him. My husband, who is larger and taller than us, sat in it and said it didn't feel bad...but he was unsure how comfortable it would be sitting in it for longer stretches of time. So...your mileage may vary.
Overall, this was exactly what we were looking for and precisely what we expected for this product, so we're happy. I will update this review should anything change.
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Reviewed in the United States on May 3, 2019