SKU: 11468571229

Mouse ACTN1 (Alpha-actinin-1) ELISA Kit

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Description

Mouse ACTN1 (Alpha-actinin-1) ELISA KitThe Mouse ACTN1 (Alpha actinin 1) ELISA Kit measures Actn1 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Actn1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection

The Mouse ACTN1 (Alpha-actinin-1) ELISA Kit measures Actn1 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Actn1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Actn1 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Actn1 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 11468571229

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4.6 ★★★★★
Based on 24 reviews
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Product Reviews
J
James J Clement
Carnegie, US
★★★★★ 5
Phoebe approved! 3 months and still going!!
Color: Hammer, Size: Large, Color: Hammer, Size: Large
I have 2 dogs--a chocolate lab and a pittie mix. Normally, they shred their toys within minutes/hours if not days, but this one has lasted for over 3 months! It is well loved and starting to show some wear, but I am super impressed with how long this one has lasted. It has a couple holes in it but they haven't disemboweled it. The squeeker didn't last too long, but they never do.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 7, 2026
K
K. Mair
Charlottesville, US
★★★★★ 3
Good for modest chewers who don't destroy toys.
Color: Hammer, Size: Large
We have four dogs, one of whom is a super chewer. Unfortunately, this toy wasn't even durable enough to stand up to our mid-level chewer. She had the squeaker out of this in mere moments, and the rope was untied and in shreds. We had to basically remove the squeaker from her, then clip the rope from the toy so she didn't try to eat it. Now, don't get me wrong. She's still having a great time with this toy, even as she whips it around and tosses stuffing everywhere. This is definitely sized for medium to large dogs, but I would only recommend this to dogs who don't like to destroy their toys, but instead just like to squeak them and maybe play some light tug. For those dogs, this will be a great option. For super chewers, this will last only minutes and I would not recommend it.
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Reviewed in the United States on March 24, 2026
D
Deana
West Palm Beach, US
★★★★★ 5
Fun Toy for Fun Times
Color: Hammer, Size: Large
Perfect fun toy for my dog! I bought this squeaky plush toy for my dog and it has been a huge hit! The design is adorable — like a little toolbox toy — and the material feels soft yet durable enough to handle lots of chewing. The squeaker sound gets my dog’s attention every time and keeps him entertained for ages. It’s a great size for playtime and fetch, and so far it’s holding up really well against rough play. Highly recommend this if you’re looking for a fun and engaging toy for your furry friend! She chews it up and has a good time. Good price.
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Reviewed in the United States on February 8, 2026
A
Amelia Love
Charlottesville, US
★★★★★ 5
Very durable for a plush toy
Color: Hammer, Size: Large
My GSD husky mix loves this toy. She is "working on it" as I write. Her first call to action with any new toy is to get the squeaker out. This toy is well made enough so that she has not been able to reverse engineer it immediately (as she does with cheap dog toys). She seems to enjoy chewing on the handle making it squeak, which is pretty cute. The toy itself is bigger than I thought it would be which is nice, as my dog is bigger too. It is soft and well stuffed. The rope on the end will make it a great tug toy too. Right now it has my dog's full attention so we shall see how long it lasts. Overall, while not indestructible, it's definitely better than 90% of the toys we've gotten her so far in terms of durability. Great product!
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Reviewed in the United States on January 22, 2026
J
J. Sutcliffe
Birmingham, US
★★★★★ 4
Seems sturdy so far
Color: Screwdriver, Size: Large
So far my boys haven't ripped this apart, which is a good sign. They like to play tug with it and the squeaker is engaging (my pittie loves squeakers). Here's hoping it continues to last with my 2 tough chewers.
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Reviewed in the United States on December 31, 2025

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