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Description
Rat HIF1A (Hypoxia-inducible Factor 1Α) ELISA KitThe Rat (HIF1A) Hypoxia inducible Factor 1 ELISA Kit measures Hypoxia Inducible Factor 1 alpha in Rat samples. The plate has been pre coated with Rat HIF1A antibody. HIF1A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HIF1A Antibody is added and binds to HIF1A in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HIF1A antibody. After incubation unbound Streptavidin HRP is
The Rat (HIF1A) Hypoxia-inducible Factor 1Α ELISA Kit measures Hypoxia-Inducible Factor 1 alpha in Rat samples. The plate has been pre-coated with Rat HIF1A antibody. HIF1A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HIF1A Antibody is added and binds to HIF1A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HIF1A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HIF1A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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