SKU: 13212439375

Human S1P (Sphingosine 1 Phosphate) ELISA Kit

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Description

Human S1P (Sphingosine 1 Phosphate) ELISA KitThe Human S1P (Sphingosine 1 Phosphate) ELISA Kit measures S1P in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with S1P. S1P in the sample or standard competes with a fixed amount of biotin labeled S1P for sites on a pre coated antibody specific to S1P. Free components are washed off. After

The Human S1P (Sphingosine 1 Phosphate) ELISA Kit measures S1P in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with S1P. S1P in the sample or standard competes with a fixed amount of biotin-labeled S1P for sites on a pre-coated antibody specific to S1P. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of S1P in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

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SKU: 13212439375

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4.1 ★★★★★
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eve
Pawtucket, US
★★★★★ 5
👍👍👍
Format: Paperback
Great book
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 18, 2025
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JAN
Louisville, US
★★★★★ 5
Excellent resource for students
Format: Paperback
The practice tests are extremely helpful and are easy to implement in our study schedule.
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Reviewed in the United States on August 14, 2024
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Anonymous
Houston, US
★★★★★ 5
Great practice
Format: Paperback
Excellent complement to the Foundations book
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Reviewed in the United States on August 26, 2024
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Erik
Carnegie, US
★★★★★ 5
This is the book you are looking for.
Format: Paperback, Format: Paperback
After searching through about four different vocab books, I finally found one I can truly vouch for: Robert's Extremely Nerdy Guide to Digital SAT Vocabulary. Robert is actually my daughter’s SAT tutor and recommended this book. At the time, my daughter had almost given up on the vocab portion of the SAT—there were only 7 weeks left to the test date (we’re now down to just 2 weeks). Honestly, what can you achieve in that short time? Still, I decided to buy it—what did we have to lose? Because I’d already gone through so many SAT vocab books, I knew a good one when I saw it. Just flipping through the first few pages convinced me this was the right fit, and my daughter immediately got to work. The book starts with 6 sections on word roots, then moves into 26 sections of similar-meaning words. Each chapter includes a matching activity (which my daughter finds essential for retention) and ends with SAT-style practice questions. Since using this book, she spends much more time memorizing and testing words. I only wish we had discovered it earlier so she could have had more time to digest and apply what she’s learning. Before this, she was getting about 50% of vocab questions right on a good day; now, after just over a month with this book, she’s averaging 80–90%. As a side note, I’m not a native English speaker, and I’ve also learned so many words by reviewing her answers. Almost every word I’ve come across in the past and thought, “I should look this up someday” seems to be in this book—a testament to why the SAT brought vocabulary back for the digital test. This book has served my family extremely well, and I plan to start my youngest child on it earlier. Get this book, and start early.
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Reviewed in the United States on September 28, 2025
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John M McDowell
Massapequa, US
★★★★★ 5
Fantastic categories for SAT vocab
Robert’s vocab book has been a fantastic addition to my tutoring resources. My students love the ease of progression through the short chapters. It is helping tremendously with vocabulary comprehension in addition to his mock tests.
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Reviewed in the United States on March 29, 2025

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