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Description
Rat MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B) ELISA Kit (Small Sample)The Rat MAP1LC3B (Microtubule associated proteins 1A 1B light chain 3B) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat MAP1LC3B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat MAP1LC3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
The Rat MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MAP1LC3B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MAP1LC3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MAP1LC3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MAP1LC3B in the samples is then determined by comparing the OD of the samples to the standard curve.
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