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Description
Human IL-1A (Interleukin 1Α) ELISA KitThe Human (IL 1A) Interleukin 1 ELISA Kit measures Interleukin 1alpha in samples. The plate has been pre coated with Human IL1A antibody. IL1A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL1A Antibody is added and binds to IL1A in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL1A antibody. After incubation unbound Streptavidin HRP is washed away during a washing
The Human (IL-1A) Interleukin 1Α ELISA Kit measures Interleukin 1alpha in samples. The plate has been pre-coated with Human IL1A antibody. IL1A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL1A Antibody is added and binds to IL1A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL1A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IL1A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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