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Description
Human ITGB1 (Integrin Beta-1) ELISA KitThe Human (ITGB1) Integrin Beta 1 ELISA Kit measures Integrin Beta 1 in samples. The plate has been pre coated with Human ITGB1 antibody. ITGB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ITGB1 Antibody is added and binds to ITGB1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ITGB1 antibody. After incubation unbound Streptavidin HRP is washed away during a
The Human (ITGB1) Integrin Beta-1 ELISA Kit measures Integrin Beta-1 in samples. The plate has been pre-coated with Human ITGB1 antibody. ITGB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ITGB1 Antibody is added and binds to ITGB1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ITGB1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ITGB1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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