Human anti-HSPA5 (anti-Heat shock protein family A member 5) ELISA Kit

Human anti-HSPA5 (anti-Heat shock protein family A member 5) ELISA KitThe Human anti HSPA5 (anti Heat shock protein family A member 5) ELISA Kit measures HSPA5 Ab in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash

The Human anti-HSPA5 (anti-Heat shock protein family A member 5) ELISA Kit measures HSPA5-Ab in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with HSPA5-Ab bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of HSPA5-Ab in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.