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Description
Human TFF1 (Trefoil Factor 1) ELISA KitThe Human (TFF1) Trefoil Factor 1 ELISA Kit measures Trefoil Factor 1 in samples. The plate has been pre coated with Human TFF1 antibody. TFF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TFF1 Antibody is added and binds to TFF1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated TFF1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
The Human (TFF1) Trefoil Factor 1 ELISA Kit measures Trefoil Factor 1 in samples. The plate has been pre-coated with Human TFF1 antibody. TFF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TFF1 Antibody is added and binds to TFF1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TFF1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TFF1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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