Human ACAT1 (Acetyl-CoA acetyltransferase, mitochondrial) ELISA Kit

Human ACAT1 (Acetyl-CoA acetyltransferase, mitochondrial) ELISA KitThe Human ACAT1 (Acetyl CoA acetyltransferase, mitochondrial) ELISA Kit measures ACAT1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ACAT1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Human ACAT1 (Acetyl-CoA acetyltransferase, mitochondrial) ELISA Kit measures ACAT1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ACAT1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with ACAT1 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of ACAT1 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.