Human CCL21/6CKINE (C-C motif chemokine 21) ELISA Kit

Human CCL21/6CKINE (C-C motif chemokine 21) ELISA KitThe Human CCL21 6CKINE (C C motif chemokine 21) ELISA Kit measures CCL21 6Ckine in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CCL21 6Ckine antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Human CCL21/6CKINE (C-C motif chemokine 21) ELISA Kit measures CCL21/6Ckine in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CCL21/6Ckine antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with CCL21/6Ckine bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of CCL21/6Ckine in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.