8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit

8-OHdG (8-Hydroxydeoxyguanosine) ELISA KitThis 8 Hydroxydeoxyguanosine ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Universal 8 OHdG. During the reaction, Universal 8 OHdG in the sample or standard competes with a fixed amount of Universal 8 OHdG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 8 OHdG. Excess conjugate and unbound sample or standard are washed away, and Avidin

This 8-Hydroxydeoxyguanosine ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 8-OHdG. During the reaction, Universal 8-OHdG in the sample or standard competes with a fixed amount of Universal 8-OHdG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 8-OHdG. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm  ± 2 nm. The concentration of Universal 8-OHdG in tested samples can be calculated by comparing the OD of the samples to the standard curve.