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Description
Human HSPA4 (Heat Shock 70kDa Protein 4) ELISA KitThe Human (HSPA4) Heat Shock 70kDa Protein 4 ELISA Kit measures Heat Shock 70kDa Protein 4 in samples. The plate has been pre coated with Human HSPA4 antibody. HSPA4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HSPA4 Antibody is added and binds to HSPA4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HSPA4 antibody. After incubation unbound Streptavidin HRP is
The Human (HSPA4) Heat Shock 70kDa Protein 4 ELISA Kit measures Heat Shock 70kDa Protein 4 in samples. The plate has been pre-coated with Human HSPA4 antibody. HSPA4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HSPA4 Antibody is added and binds to HSPA4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HSPA4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human HSPA4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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