Human OXCT1 (3-oxoacid CoA-transferase 1) ELISA Kit

Human OXCT1 (3-oxoacid CoA-transferase 1) ELISA KitThe Human OXCT1 (3 oxoacid CoA transferase 1) ELISA Kit measures OXCT1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti OXCT1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated

The Human OXCT1 (3-oxoacid CoA-transferase 1) ELISA Kit measures OXCT1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti OXCT1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with OXCT1 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of OXCT1 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.