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Ships within 48 hours · Estimated delivery Jul 26 - Jul 31
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Description
Rabbit CNTF (Ciliary Neurotrophic Factor) ELISA KitThe Rabbit CNTF (Ciliary Neurotrophic Factor) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rabbit CNTF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit CNTF and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each
The Rabbit CNTF (Ciliary Neurotrophic Factor) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit CNTF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit CNTF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit CNTF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit CNTF. You can calculate the concentration of Rabbit CNTF in the samples by comparing the OD of the samples to the standard curve.
Shipping Notes
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Exchange/Return Notes
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