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Ships within 48 hours · Estimated delivery Jul 10 - Jul 15
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Description
Rat PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA KitThe Rat PACAP 27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Rat PACAP 27. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Rat PACAP 27. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
The Rat PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat PACAP-27. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Rat PACAP-27. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PACAP-27 in the samples is then determined by comparing the OD of the samples to the standard curve.
Shipping Notes
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Exchange/Return Notes
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