SKU: 62096017039

Equine Anti-TT (Tetanus Toxin/Toxoid) IgG ELISA Kit

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Ships within 48 hours · Estimated delivery Sep 18 - Sep 23

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Description

Equine Anti-TT (Tetanus Toxin/Toxoid) IgG ELISA KitThe Equine Anti TT (Tetanus Toxin Toxoid) IgG ELISA Kit measures anti TT IgG in Equine Serum, Plasma. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP detection antibody, then it binds

The Equine Anti-TT (Tetanus Toxin/Toxoid) IgG ELISA Kit measures anti-TT IgG in Equine Serum, Plasma. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with anti-TT IgG bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of anti-TT IgG in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 62096017039

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