PGE1 (Prostaglandin E1) ELISA Kit
SKU: 63881149095

PGE1 (Prostaglandin E1) ELISA Kit

Sale price$202.50 Regular price$225.00
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Description

PGE1 (Prostaglandin E1) ELISA KitThe PGE1 (Prostaglandin E1) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with PGE1. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to PGE1. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme substrate

The PGE1 (Prostaglandin E1) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGE1. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to PGE1. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGE1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 63881149095

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