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Description
Human PCK2 ELISA KitThe Human PCK2 ELISA Kit measures Phosphoenolpyruvate carboxykinase,GTP, mitochondrial in samples. The plate has been pre coated with Human PCK2 antibody. PCK2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PCK2 Antibody is added and binds to PCK2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PCK2 antibody. After incubation unbound Streptavidin HRP is washed away
The Human PCK2 ELISA Kit measures Phosphoenolpyruvate carboxykinase,GTP, mitochondrial in samples. The plate has been pre-coated with Human PCK2 antibody. PCK2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PCK2 Antibody is added and binds to PCK2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PCK2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PCK2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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