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Ships within 48 hours · Estimated delivery Jul 29 - Aug 3
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Description
SPH (Sphingomyelin) ELISA KitThe SPH (Sphingomyelin) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with SPH. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to SPH. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme substrate
The SPH (Sphingomyelin) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SPH. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to SPH. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SPH in the samples is then determined by comparing the OD of the samples to the standard curve.
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
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