Human TIMP-4 (Tissue Inhibitors of Metalloproteinase 4) ELISA Kit
SKU: 73402052186

Human TIMP-4 (Tissue Inhibitors of Metalloproteinase 4) ELISA Kit

Sale price$202.50 Regular price$225.00
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Description

Human TIMP-4 (Tissue Inhibitors of Metalloproteinase 4) ELISA KitThe Human TIMP 4 (Tissue Inhibitors of Metalloproteinase 4) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human TIMP 4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TIMP 4 and Avidin Horseradish Peroxidase (HRP) conjugate are added

The Human TIMP-4 (Tissue Inhibitors of Metalloproteinase 4) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TIMP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TIMP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TIMP-4. You can calculate the concentration of Human TIMP-4 in the samples by comparing the OD of the samples to the standard curve.

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SKU: 73402052186

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michael parsley
Port Orchard, US
★★★★★ 5
Super tough
Color: Red Warthog
My dogs love these toys!!!
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Reviewed in the United States on February 10, 2026
A
Verified Purchase
Amazon Customer
Lowell, US
★★★★★ 5
Long lasting play toy!
Color: Multicolor
Tuffy toys in general are great and they are a pretty good deal on Amazon. They are not indestructible, and if your dogs use them as chew toys they will eventually break through. My dogs have had this Armadillo since Christmas (and they do chew on it some) and the only thing missing is an ear. They like to play tug-of-war with it a lot. This is a bigger toy, but even my forty pound dog plays with it. I would not recommend this toy for dogs smaller than Border Collie size. You can make these Tuffy toys last longer if you don't allow your dogs to chew them--when your dogs starts chewing on non-chew toys trade them for a chew toy and when you catch your dog chewing on a chew-approved toy reward them for chewing. Eventually your dog will catch on and realize that if they want to chew there are certain toys that are allowed for that. In addition to being a great play toy this is a pretty cute toy too!
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Reviewed in the United States on June 30, 2015
A
Verified Purchase
Amazon Customer
Louisville, US
★★★★★ 1
Not Tuff
Color: Black Yellow Phrog, Color: Black Yellow Phrog
Absolutely terrible and not “tuff” at all. My chocolate lab had this thing destroyed in less than two hours. I would not recommend these for the price.
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Reviewed in the United States on April 11, 2026
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Verified Purchase
Carrie Ann Moore
New York, US
★★★★★ 3
Cute but Not Durable
Color: 2PK Green Leaping Phrog
⭐️⭐️⭐️⭐️ Cute but Not Durable Super cute toy—my dogs loved it right away! Unfortunately, they chewed the head off within a few hours. I ordered a second one because they were so into it, but the same thing happened again. I won’t be ordering a third, but they still play with their two headless frogs. So while it’s not the most durable, it definitely kept them entertained!
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Reviewed in the United States on June 9, 2025
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Verified Purchase
Lauren
Draper, US
★★★★★ 3
Puppy loves! Supposed to be part of a set though??
Style: Coconut Tree
Bought this simply because we named our puppy Coco and was on a roll ordering her toys… she ended up absolutely LOVING this tiny palm tree!! Maybe because of the size and she was able to really handle it, she takes it everywhere with her and loves the squeaker. HOWEVER, the item is a lot smaller than I expected when ordering for almost $10 and the plastic packaging it came in says “not intended for individual sale, part of a pack” so I would not purchase from this seller again. I will maybe try to find this included with the intended set of toys.
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Reviewed in the United States on May 28, 2022

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