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Ships within 48 hours · Estimated delivery Jul 27 - Aug 1
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Description
FLG (Filaggrin) ELISA Kit (Small Sample)The FLG (Filaggrin) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human FLG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human FLG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those
The FLG (Filaggrin) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FLG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FLG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FLG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FLG in the samples is then determined by comparing the OD of the samples to the standard curve.
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Exchange/Return Notes
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