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Ships within 48 hours · Estimated delivery Jul 29 - Aug 3
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Description
Rabbit BAFF/CD257 (B-Cell Activating Factor) ELISA KitThe Rabbit BAFF CD257 (B Cell Activating Factor) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rabbit BAFF CD257. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit BAFF CD257 and Avidin Horseradish Peroxidase (HRP) conjugate are added
The Rabbit BAFF/CD257 (B-Cell Activating Factor) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit BAFF/CD257. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit BAFF/CD257 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit BAFF/CD257, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit BAFF/CD257. You can calculate the concentration of Rabbit BAFF/CD257 in the samples by comparing the OD of the samples to the standard curve.
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
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