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Description
Human Anti-Semaglutide Neutralizing antibody ELISA KitThe Human Anti Semaglutide Neutralizing antibody ELISA Kit measures Anti Semaglutide Neutralizing in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with anti Semaglutide Neutralizing antibody antibody. Sample or standard completely mixes with fixed amount of biotin labeled Semaglutide
The Human Anti-Semaglutide Neutralizing antibody ELISA Kit measures Anti-Semaglutide Neutralizing in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with anti Semaglutide Neutralizing antibody antibody. Sample or standard completely mixes with fixed amount of biotin-labeled Semaglutide Neutralizing antibody working solution and also add into each well. Semaglutide Neutralizing antibody in the sample or standard competes with biotin-labeled Semaglutide Neutralizing antibody to bind with antibody precoated on the microplate. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of Semaglutide Neutralizing antibody in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
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Exchange/Return Notes
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