Rabbit ENA-78 (Epithelial Neutrophil Activating Peptide 78) ELISA Kit

Rabbit ENA-78 (Epithelial Neutrophil Activating Peptide 78) ELISA KitThe Rabbit ENA 78 (Epithelial Neutrophil Activating Peptide 78) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rabbit ENA 78. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit ENA 78 and Avidin Horseradish Peroxidase (HRP) conjugate are

The Rabbit ENA-78 (Epithelial Neutrophil Activating Peptide 78) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit ENA-78. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit ENA-78 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit ENA-78, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit ENA-78. You can calculate the concentration of Rabbit ENA-78 in the samples by comparing the OD of the samples to the standard curve.