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Description
Human HMGB-1 (High Mobility Group Protein B1) ELISA KitThe Human (HMGB 1) High Mobility Group Protein B1 ELISA Kit measures High Mobility Group Protein B1 in samples. The plate has been pre coated with Human HMGB1 antibody. HMGB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HMGB1 Antibody is added and binds to HMGB1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HMGB1 antibody. After incubation unbound Streptavidin HRP
The Human (HMGB-1) High Mobility Group Protein B1 ELISA Kit measures High Mobility Group Protein B1 in samples. The plate has been pre-coated with Human HMGB1 antibody. HMGB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HMGB1 Antibody is added and binds to HMGB1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HMGB1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human HMGB1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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Exchange/Return Notes
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