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Description
Mouse PEDF (PIgMent Epithelium-derived Factor) ELISA KitThe Mouse (PEDF) PIgMent Epithelium derived Factor ELISA Kit measures Pigment Epithelium Derived Factor in Mouse samples. The plate has been pre coated with Mouse SERPINF1 antibody. SERPINF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SERPINF1 Antibody is added and binds to SERPINF1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SERPINF1 antibody. After incubation
The Mouse (PEDF) PIgMent Epithelium-derived Factor ELISA Kit measures Pigment Epithelium-Derived Factor in Mouse samples. The plate has been pre-coated with Mouse SERPINF1 antibody. SERPINF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SERPINF1 Antibody is added and binds to SERPINF1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SERPINF1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse SERPINF1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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