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Description
VEGF R2/Flk-1/KDR His Tag Protein, Rhesus macaqueProduct Specification Species Rhesus macaque Synonyms KDR,CD309,FLK1,VEGFR,VEGFR2 Accession F7E313 Amino Acid Sequence Ala20 Glu764, with C terminal His Expression System HEK293 Molecular Weight 100 130kDa (Reducing) Purity 95% by SDS PAGE & SEC HPLC Endotoxin <0. 1EU g Conjugation Unconjugated Tag His Tag Physical Appearance Lyophilized Powder Storage Buffer PBS, PH7. 4, 5% trehalose Reconstitution Reconstitute at 0. 1 1 mg ml according to the size
Product Specification
| Species | Rhesus macaque |
| Synonyms | KDR,CD309,FLK1,VEGFR,VEGFR2 |
| Accession | F7E313 |
| Amino Acid Sequence | Ala20-Glu764, with C-terminal His |
| Expression System | HEK293 |
| Molecular Weight | 100-130kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE & SEC-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1.Lian, L. et al. (2019) BMC Cancer. 19:183. |
Background
Vascular endothelial Growth Factor Receptor 2 (VEGFR2), also known as FLK-1, KDR, and CD309, is a type I single-pass membrane receptor，and is one of the subtypes of VEGFR. VEGF receptors are receptors for vascular endothelial growth factor (VEGF). Mature VEGFR2 contains a 745 amino acid extracelluar domain with seven immunoglobulin-like repeats, 21 transmembrane domain and 571 cytoplasmic domain. Within the extracellular domain, human VEGFR2 shares 80% homology with that of mouse and rat. VEGFR-2 appears to mediate almost all of the known cellular responses to VEGF. In addition, VEGFR2 is able to interact with HIV-1 extracellular Tat protein upon VEGF activation, and seems to enhance angiogenesis in Kaposi's sarcoma lesions.
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