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Description
Mouse WND ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Wilson disease protein (WND) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and then to yellow by acid. The intensity of the color is positively correlated with the presence of Wilson disease protein (WND) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Wilson disease protein ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Copper transporter protein beta chain (ATP7B), also known as Wilson disease protein (WND), or ATP7B protein, is a copper-transporting P-type ATPase encoded by the ATP7B gene. This protein is located in the trans-Golgi network of the liver and brain and balances copper levels in the body by excreting excess copper into bile and plasma. Inherited disorders of the ATP7B gene may cause Wilson disease, a disorder in which copper accumulates in tissues, leading to neurological or psychiatric disorders and liver disease. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.5 ★★★★★
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Product Reviews
★★★★★ 5
Overkill In A Good Way
Size: 1.5FT, Size: 1.5FT
Bought this UGREEN Cat 8 Ethernet cable to connect my router to my modem, and it works perfectly. Build quality is excellent — thick braided but bendable cable, solid connectors, and it clicks in snug. Definitely feels more durable than the cheap cables you usually get.
Speeds are stable with no drops or lag, and setup was plug-and-play. The 1.5 ft length is perfect for short runs and keeps everything looking neat instead of having extra cable everywhere.
Is Cat 8 probably more than most people need? Yeah, as it is more than enough for home internet speeds of 1 to 2 gigs. But for its value, it’s nice to know it’s future-proof for many homeowners and the speeds currently offered by today’s ISPs. Would absolutely buy again.
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Reviewed in the United States on January 31, 2026
★★★★★ 5
Glad I Bought the Better Cable
Size: 50FT
I originally looked at some cheaper Ethernet cables, but my son talked me into spending a little more for this one. I’m glad I listened.
The cable feels well made, and the braided design seems sturdy. I bought it for my fiber internet setup so I could have a reliable wired connection to my laptop.
It has worked perfectly. I plugged one end into the router and the other end into my adapter, and that was it. No issues, no disconnects, and no problems.
Sometimes it is worth paying a little more for something you only want to buy once. This cable was worth it.
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Reviewed in the United States on June 2, 2026
★★★★★ 5
A Must if you have Fiber internet!!!
Size: 10FT
I recently got fiber internet installed and decided to upgrade my Ethernet cable from Cat 7 to this UGREEN Cat 8 cable—mostly just because I wanted to make sure my network setup was fully up to date. I honestly wasn't expecting much of a difference, but I was surprised to see over a 20% increase in speed after swapping out the cable.
The cable itself is extremely high quality. The braided exterior feels durable and premium, and the connectors are solid and well-made. Everything about it gives the impression of a quality product designed to last.
Whether the speed improvement was due to my previous cable or not, I'm very happy with the results. For anyone upgrading their network, especially with fiber internet, this was a worthwhile upgrade and well worth the money.
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Reviewed in the United States on June 7, 2026
★★★★★ 5
UGREEN Cat 8 6FT Braided – Finally a short cable that actually hits 40 Gbps in real life
Size: 6FT
⭐⭐⭐⭐⭐
UGREEN Cat 8 6FT Braided – Finally a short cable that actually hits 40 Gbps in real life
I have a 10 Gbit NAS (Synology DS1823xs+) sitting right behind my gaming PC, and every “Cat8” cable I tried before this one was either stiff boat-anchor thick or straight-up lied about speed. This UGREEN 6 ft braided version is the first one that actually delivers.
Real-world tested speeds
iperf3 PC ↔ NAS: sustained 39.4 Gbps bidirectional (that’s ~4.9 GB/s)
Large 4K/8K file transfers: 4.7–4.8 GB/s steady, no drops
Zero packet errors after 48 hours of torture testing
Build quality & practicality
Flat braided jacket is super flexible, looks premium, and doesn’t turn into a tangled mess like round Cat8 cables
Gold-plated contacts, double shielding, and it passes a Fluke DSX-8000 Cat8 Channel test (yes, I’m that guy)
Perfect 6 ft length, no excess coil behind the desk causing signal reflections
Snagless boot actually works, no more broken clips
Why I love it for desktop use
Runs cool even at full 40 Gbps load (barely warm)
Way less stiff than the usual shielded Cat8 firehoses, easy to route under a desk or through a cable sleeve
Latency is stupid low: 0.22 ms average to my NAS vs 0.45 ms on my old Cat6a round cable
Only “con” (and it’s tiny): it’s flat, so if you’re one of those people who religiously wants perfectly round cables for aesthetics, you might not like it. Everyone else on planet Earth will think it looks cleaner anyway.
For $14, this is the best short-hop 40 Gbps cable on Amazon, bar none. Already replaced every 1–10 ft run in my office with these. If you have 10G+ gear sitting close together and you’re still using Cat6 or stiff Cat8, do yourself a favor and grab a couple. You’ll instantly feel the difference.
10/10 – UGREEN absolutely crushed it with this one.
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Reviewed in the United States on December 12, 2025
★★★★★ 5
Still the best ethernet cable out there right now
Size: 6FT
I ordered several of these cables a couple years ago and they are still the best cables I have ever purchased. The Cat8 speed rating is great. These are very well built with the outer covering and the quality connectors and covers over them. Fast data transfer wtih Cat8. I am ordering more tonight to replace some other cables as these just look, feel and work premium.
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Reviewed in the United States on May 12, 2026
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