SKU: 19457208937

Human IL-4 High Sensitivity ELISA Kit

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Description

Human IL-4 High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti human IL 4 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL 4 existing in the

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti-human IL-4 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-4 existing in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-4 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, IL-4 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.03pg/ml

Detection Range: 0.25-16 pg/ml

Recovery Range: 94-105%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 4 (IL-4) is a monomeric cytokine of about 18-20kda that can induce naive helper T cells (Th0 cells)Differentiation into Th2 cells. After being activated by IL-4, Th2 cells then secrete IL-4 in a positive feedback manner. The cell type that secretes IL-4 to induce Th0 cell differentiation has not yet been identified, but recent studies have shown that basophils may be this effector cell. IL-4 is closely related to IL-13 and has similar functions.

IL-4 can also promote rhabdomyosarcoma to undergo mitosis, dedifferentiation and metastasis. It can be observed that IL-4 and other Th2 cytokines are involved in airway inflammation in allergic asthma patients.

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SKU: 19457208937

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Tiffany
Lexington, US
★★★★★ 5
Must have for rosacea sensitive skin!
Style: Tolerance Control Soothing Skin Recovery Cream
I have fair, sensitive skin & since being in peri-menopause I suddenly have rosacea. This cream is SO gentle on the skin but so instantly calming. It hydrates so well & a little goes a long way. I will be buying this over & over from now on!
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Reviewed in the United States on April 15, 2026
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Amazon Customer
Lowell, US
★★★★★ 5
Great for Sensitive Skin
Style: Tolerance Control Soothing Skin Recovery Cream
I have very sensitive skin and have tried everything to try and prevent rosacea flairs. I recently came across this brand and I absolutely love this recover cream. I consider this now the holy Grail for Rosacea and sensitive skin. It makes my skin feel hydrated and non-reactive. It is soothing and I just cannot get enough of it. It is pricy but it is worth it to not have my face constantly feeling like its burning or irritated.
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Reviewed in the United States on April 2, 2026
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Kindle Customer
Lexington, US
★★★★★ 5
Wonderful recovery Cream
Style: Tolerance Control Soothing Skin Recovery Cream
The cream is very soothing on the skin, it is all that it claims to do. After a couple of uses, my skin cleared up of all the dryness and redness. It is gentle and effective on the skin. Its very good value for money. For anyone with sensitive skin its very beneficial. I will recommend it.
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Reviewed in the United States on May 20, 2026
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Kindle Customer
Battle Creek, US
★★★★★ 4
Great product for sensitive skin
Style: Tolerance Control Soothing Skin Recovery Cream
I have sensitive skin that is constantly getting irritated by moisturizers/seemingly simple skin care products. I read a few blogs/articles/reviews on different products others have tried for similar issues and this one came up, so I decided to give it a try. It’s nice and light and goes on smoothly. Skin feels moisturized and does not get irritated, so that’s a win! The only reason I took off 1 star is it leaves my skin a bit oily for my liking, but that could just be my skin.
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Reviewed in the United States on February 9, 2026
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Amberkd
Port Orchard, US
★★★★★ 5
perfect for sensitive skin
Style: Tolerance Control Soothing Skin Recovery Cream
This provided perfect therapy for sensitive skin. IT WORKS!
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Reviewed in the United States on May 19, 2026

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