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Description
Phospho-Akt Substrate (RXRXXST) Recombinant Rabbit mAb (S-2624-20)Product Specification Host Rabbit Antigen Phospho Akt Substrate (RXRXXST) Immunogen Synthetic Peptide Clone Number S 2624 20 Antibody Type Recombinant mAb Isotype IgG Application ELISA, WB Reactivity Hu, Ms Purification Protein A Concentration 2 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 20 C as supplied
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Akt Substrate (RXRXXST) |
| Immunogen | Synthetic Peptide |
| Clone Number | S-2624-20 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ELISA, WB |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
Background
The Phospho-Akt Substrate (RXRXXS/T) motif is the core sequence specifically recognized and phosphorylated by protein kinase B (Akt). In this motif, R represents arginine, X represents any amino acid, and S/T represents the phosphorylation site serine or threonine. The characteristic arginine residues (RXR) are responsible for specific binding to the catalytic domain of Akt, while the C-terminal serine or threonine (S/T) is the site where Akt catalyzes the phosphorylation reaction. This highly conserved motif is widely found in various downstream substrate proteins of Akt, such as GSK-3β and FOXO transcription factors, and serves as a central hub regulating key signaling pathways involved in cell survival, proliferation, and metabolism. Detecting the phosphorylation level of this motif directly reflects the in vivo activity status of Akt kinase.
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