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Description
Mouse IL-31 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect them by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with ice-cold PBS and resuspend them in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes, remove the supernatant, or store at -20°C or -80°C, but avoid repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000×g for 20 minutes, and remove the supernatant for analysis. Pre-Assay Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the gradient working solution of the standard: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration is 400 pg/mL). Then dilute to the following concentrations: 400 pg/mL, 200 pg/mL, 100 pg/mL, 50 pg/mL, 25 pg/mL, 12.5 pg/mL, 6.25 pg/mL, and 0 pg/mL. Serial Dilution Method: Add 500 μL of universal diluent to each of seven EP tubes. Pipette 500 μL of the 400 pg/mL standard working solution into the first EP tube and mix thoroughly to make a 200 pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 31 (IL-31) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Interleukin 31 (IL-31) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Interleukin 31 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-31 (IL-31) is a protein encoded by the IL31 gene located on chromosome 12. It has also been identified as a key player in several chronic inflammatory diseases, including atopic dermatitis. It is produced by various cell types, including type 2 helper (TH2) T cells. It signals through a receptor complex composed of IL-31RA and oncostatin M receptor β (OSMRβ), expressed on immune and epithelial cells. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 6.25-400pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids |
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4.0 ★★★★★
Based on 29 reviews
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Product Reviews
★★★★★ 5
Recomendado
Color: 6638: Gold band & Green dial
Excelente! Me gustó mucho y si cumplió con mis expectativas
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 4, 2026
★★★★★ 5
Exelente
Color: 6638: Gold strap & Blue dial
Ha salido muy bueno
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 8, 2026
★★★★★ 5
Compared to g-shock?
Color: Black/Yellow
Firstly random public reviews can be comical…”it’s has a battery, terrible, One star” or “I don’t like yellow, one star” omg peoples do better. I’ll review this while comparing to an older style of g-shock from the same era. I’ll start by saying this timex rocks, for me in my mid 50’s it’s so retro and nastalgic, brings back memories, I may have even owned this model back in the day. What’s great about it- the display, larger digits than the typical g-shock and they are super crisp, clear and easy to read. The indiglo is awesome. This is the most accurate quartz watch I have aside from the atomic g-shocks that adjust themselves daily. I prefer a chunky watch and the g-shocks do a better job in that department. i feel this watch is rugged, but I’d still give the nod to g-shock in this department. 200M water resistance is the gold standard and this watch has it which I love for peace of mind for any water based activities. This is a 5 star watch at a great price. I still love some aspects of a g-shock better but its just preference.
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Reviewed in the United States on February 11, 2026
★★★★★ 5
Everybody loved the high-tech style and amazing functionality
Color: Black/Yellow
I'm not a Timex fanboy, and I've had my fair share of problems with the Expedition line, so this review is my honest opinion as a man who's worn Armitrons and Timexes since I was 10.
I remember in 1992 when the Timex Ironman Triathlon with Indiglo was first launched - My older brother got one, then me and my neighbor friends. Everybody loved the high-tech style and amazing functionality. Even Bill Clinton was rock'n this watch, for those who don't remember.
Quite simply, this is the perfect watch at a great price, in my opinion. Perfect as a gift for any man, and I think the original black/olive drab/yellow coloration is still the coolest. In this day and age of smartphones, it still pays to have this watch. The alarm is less annoying sounding than a cellphone, and doesn't wake up the entire house. To turn it off, just tap the watch, no more reaching for the phone or alarm clock. Timer comes in handy for anything; timing my kids' timeouts when they get in trouble, 15 minutes room clean-up times, etc. Chrono is fun for anything you wanna use a stop-watch for. I set the second time zone for Vietnam time. It really is a million times easier to use this watch for all of its functions than reaching for a smartphone or alarm clock, etc. If you;ve been relying on your phone for a few years as a watch/chronograph/timer/alarm - you'll be so glad you finally got a watch like this.
I've never tried a smart-watch, however I feel those are not as necessary,durable or practical as a real watch. Other Timex watches I've used were freakin' trash - couldn't throw em away fast enough. Might as well open the box while holding it over a trash can, so the watch can fall right in when you open the box. That's my experience with the Expedition line-up.
Back to this watch: The only thing I wish this watch could do is measure activity thermogenesis (calories expended throught the day) or work as a pedometer, which some smart-watches and other devices can do. I also cannott decide if I like this new, slightly larger, heavier/thicker design of the Ironman Triathlon over the 1990s design. The 90s models styling was "tight to death'. Star-split buttons were seperated. The mode button was yellow. Overall more compact and bi-cultural;by that I mean it looked great as an athletic watch or a politician, business man wathc. This new model is bigger, better ingress/shock resistance, and more of an athletic style over all, and is a little less colorful.
I almost went with the G-shocks, but they still just look like crap and are waay to expensive. Sure, they have some better features, can survive WWIII, but I am a traveler. I need a watch that can work in the dark in any environment. The G-shocks are often using some kind of solar battery crap and rely on cathing a signal from an American automic clock in Colorado - good luck with that while your in Thailand on vacation.
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Reviewed in the United States on December 31, 2016
★★★★★ 5
Great Product. Easy to use.
Color: Black
This is the third running watch I've had and it's my first Timex. I've owned to Casio's before (one being a g-shock), but after finding my g-shock to bulky and cluttered, and loosing my other Casio I picked up from Walmart, I decided to look around to find a new durable running watch.
I run cross country and track so going from a generic stopwatch to a 30-lap chrono watch has been sweet. The lap timer is easy to use and the display is big enough to take a glance and see my split and total time side by side. The recall feature works perfectly and you even have some room to adjust the settings to configure your lap display how you like it.
My favorite thing about this Timex, is its ease of use. The only time I've had to look at a manual is when I first got the watch. The display updates as you go through the various modes to show you what the new buttons mean so there is never a question on how to set the time or recall your laps.
This watch is extremely durable. It has already taken a few shots and I've swam with it on several times. The screen and body have held up nicely and I don't foresee any problems with them in the future. The strap is great also. I really like the notch that keeps the excess strap in place.
The countdown timer and the alarm both work as expected. The timer has some different settings and is easy to set up. My only disappointment with the timer was that you can't set different intervals. Like if you want to run for five minutes and walk for two your going to have to invest a little more for a watch that allows different intervals. The alarm works well too, and the beeping successfully wakes me up in the morning, but I'm not a heavy sleeper so some may find it too quiet.
Overall this watch is durable, easy to use, and can track splits really well. The watch is a little bulky if you are used to a more minimalist watch but for me the weight was perfect, and for the price you can't go wrong with this watch.
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Reviewed in the United States on June 5, 2013
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