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Description
Mouse monoamine oxidase,MAO ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with monoamine oxidase and MAO capture antibodies. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the monoamine oxidase and MAO content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse monoamine oxidase,MAO ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Monoamine oxidases (MAO) are a family of enzymes that catalyze the oxidation of monoamines, using oxygen to remove their amine groups. They are found bound to the outer mitochondrial membrane of most cell types in the body. The first such enzyme was discovered in the liver by Mary Bernheim in 1928 and named tyramine oxidase. MAO belongs to a family of proteins called flavinamine oxidoreductases. They are important in breaking down monoamines ingested through food and also play a role in inactivating monoamine neurotransmitters. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
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4.4 ★★★★★
Based on 5 reviews
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Product Reviews
★★★★★ 2
Wrong size
Nothing wrong with it except it was too big to fit under my table so I had to return it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 28, 2026
★★★★★ 5
Keeps my pets very comfortable.
Color: Purple Fish, Size: Medium (27'' X 20'' X 0.3''), Number of Items: 4
I purchased the 4-pack for my four pets, and they absolutely loved them! The cooling mats are soft, lightweight, and really do stay cool, which has been perfect for the warmer weather. I placed them on their beds, car seats, and favorite lounging spots, and every one of my pets took to them right away.
I also love that they’re portable, washable, and made with a nice quality material that feels durable while still being comfortable. The purple fish design is adorable too! Definitely a great purchase for pet owners looking to help keep their furry babies cool and comfortable during the summer. Highly recommend!
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Reviewed in the United States on May 22, 2026
★★★★★ 5
Make your cat happy
Color: Pink Cat, Size: Medium (27'' X 20'' X 0.3''), Number of Items: 1
My cat loves this. She was always moving from spot to spot looking for a cooler place to lie. When I put this pad down, she got on it and stays there for long periods of time. The pad is actually cooler than I thought it might be. Good product.
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Reviewed in the United States on April 29, 2026
★★★★★ 3
Cool to the touch.
Color: Green Leaf, Size: Small (20'' X 16'' X 0.3''), Number of Items: 4
If definitely feels cool to the touch although still have doubts about ability to cool my dogs. I have not trained my dogs to lay on it so I’m not sure if they know how to use the cooling pads but I do see my one dog lay on it from time to time so perhaps it is effective, not sure. Cooling pad slips and it folds ups because there is no grip on the bottom side. It feels smooth and soft to the touch because of the fabric on the outside. I plan on brining it in my dog stroller in the summer and should be better than having no cooling pad. Although not as cool as an ice pack, this cooling pad doesn’t require refrigeration and always stays cool to the touch and no condensation. Not sure if it’s worth the money for the price because not sure if it works. A four pack for $32 seems reasonable but not sure if it’s worth it.
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Reviewed in the United States on August 10, 2025
★★★★★ 5
Great for thick fur kitties!
Color: Green Leaf, Size: Medium (27'' X 20'' X 0.3''), Number of Items: 1, Color: Green Leaf, Size: Medium (27'' X 20'' X 0.3''), Number of Items: 1
A summer cooling mat full of endless possibilities! Kitty 🐈⬛ imagination highly required! It’s apparently somewhat better than a large empty Amazon box! Beau highly recommends it!
****Review annotated by Beau’s mom
Beau’s mom actual review:
I purchased the medium sized cooling mat for my chunkster grey tux Beau who at first snoop, didn’t like it. After carefully flopping him on top of it and made stupid sounds, he inevitably decided to actually try it his way thus (while not a box) liked it. I was surprised that after two weeks the mat was still cool and scratch-free from his murder mittens. While he does flop on the mat from time to time, he enjoys dragging it, flipping it in a viscous cycle of who-knows what the heck is going on his mind! Either way, if I could give it 9/10 stars I would as the instructions fails to inform user of a highly kitty imagination is required. No worries tho! Beau figured this out two days into flopping on top of it.
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Reviewed in the United States on April 28, 2026