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Description
Human Lung Cancer Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) the tissue after sampling must be placed in the pre cooled (2 8 c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information (2) prepare several Petri dishes and add 4 pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue into the Petri dish,
Product Specification
| Usage |
1. Primary (1) the tissue after sampling must be placed in the pre cooled (2-8° c) sampling bottle of tissue preservation solution e, and quickly transferred to the clean laboratory for tissue processing and cell separation, taking photos and registering information (2) prepare several Petri dishes and add 4 ℃ pre cooled primary culture buffer B for standby (3) disinfect the sampling bottle, put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, and cut the tissue into a tissue block with a volume of about 1-3mm3by using ophthalmic scissors or scalpel (4) the tissue was digested with human lung cancer primary tissue digestive solution C, and the shaking digestion was carried out at 37 ℃ for 10-20min (the digestion condition was observed at any time during the digestion process) (5) take a small amount of liquid and observe it under the microscope. After observing more single cells or cell clusters below 70UM under the microscope, add three times the volume of primary culture buffer B to stop digestion (6) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation (7) Matrigel calculation: after step 6, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate (8) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃) (9) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a gel, and add human lung cancer organoid medium a (restore room temperature) for culture 2. Organoid subculture (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4  &Nbsp&Nbsp&Nbsp b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 (4) after organoids are collected, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul human lung cancer organoid culture medium a 3. Organoid cryopreservation (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid (4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube (5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage 4. Organoid resuscitation (1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube (2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting (3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min (4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g (5) resuspend the Matrigel, lay 25ul of Matrigel per well in a 24 well cell culture plate, place it in an incubator for 10-15min to form a gel, and add 500ul of human lung cancer organoid culture medium a. |
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| Description |
Composition:
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| Storage Temp. | 4° C. 3months-20° C. 1 year, see label for details |
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4.4 ★★★★★
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Product Reviews
★★★★★ 5
Satin pillowcases
Size: Standard (20" X 26"), Color: 09 - Ivory, Number of Items: 2, Size: Standard (20" X 26"), Color: 09 - Ivory, Number of Items: 2
These pillowcases are honestly a great purchase. They feel incredibly smooth and soft and I noticed a real difference in both my hair and skin! my hair is less frizzy in the morning, and my skin feels softer.
The quality is excellent for the price, and they’ve held up well after several washes without losing their softness or color. They also feel cool and comfortable throughout the night, which is a nice bonus.
Overall, I’m really happy with this purchase and would definitely recommend them to anyone looking to upgrade their sleep experience!
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Reviewed in the United States on April 28, 2026
★★★★★ 5
Great for my frizzy hair
Size: Queen (20" X 30"), Color: 06 - Black, Number of Items: 2
I got these because I really wanted something to helps tame my frizzy hair but also help with my skin. I have very sensitive skin and my break outs have reduced so much! They fit my standard pillows without an issues. The color never fades, they wash and dry well. The quality is on point. Worth it.
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Reviewed in the United States on May 17, 2026
★★★★★ 5
Great quality & colors
Size: Body (20" X 54"), Color: 03 - Beige, Number of Items: 1
Good price so is easier to buy several colors. Perfect fit for a body pillow. Super there are pillow cases to match. Soft! Great quality.
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Reviewed in the United States on May 25, 2026
★★★★★ 5
Cute, soft, and warm!
Color: Cow, Size: Throw(50"x60")
Super soft and super warm! This nice and thick blanket feels so nice when you wrap yourself up in it; and it’s just big enough to do exactly that!
I’ve washed it a few times and it comes out even softer and fluffier than when I put it in the washer; and no issues with losing fluff or thinning/tears.
I love the cow pattern too!
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Reviewed in the United States on October 25, 2025
★★★★★ 5
SOFT
Color: Red Black, Size: Twin(60"x80")
My grandson absolutely loves this blanket. It’s so soft.
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Reviewed in the United States on May 25, 2026