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Description
EphA5 His Tag Protein, HumanProduct Specification Species Human Synonyms EHK 1, EK7, BSK, EHK1, HEK7, TYRO4, Rek7, TYRO4HEK7CEK7 Accession P54756 Amino Acid Sequence Pro25 Pro573, with C terminal 10*His
Product Specification
| Species | Human |
| Synonyms | EHK-1, EK7, BSK, EHK1, HEK7, TYRO4, Rek7, TYRO4HEK7CEK7 |
| Accession | P54756 |
| Amino Acid Sequence | Pro25-Pro573, with C-terminal 10*His PASLAGCYSAPRRAPLWTCLLLCAALRTLLASPSNEVNLLDSRTVMGDLGWIAFPKNGWEEIGEVDENYAPIHTYQVCKVMEQNQNNWLLTSWISNEGASRIFIELKFTLRDCNSLPGGLGTCKETFNMYYFESDDQNGRNIKENQYIKIDTIAADESFTELDLGDRVMKLNTEVRDVGPLSKKGFYLAFQDVGACIALVSVRVYYKKCPSVVRHLAVFPDTITGADSSQLLEVSGSCVNHSVTDEPPKMHCSAEGEWLVPIGKCMCKAGYEEKNGTCQVCRPGFFKASPHIQSCGKCPPHSYTHEEASTSCVCEKDYFRRESDPPTMACTRPPSAPRNAISNVNETSVFLEWIPPADTGGRKDVSYYIACKKCNSHAGVCEECGGHVRYLPRQSGLKNTSVMMVDLLAHTNYTFEIEAVNGVSDLSPGARQYVSVNVTTNQAAPSPVTNVKKGKIAKNSISLSWQEPDRPNGIILEYEIKYFEKDQETSYTIIKSKETTITAEGLKPASVYVFQIRARTAAGYGVFSRRFEFETTPVFAASSDQSQIPGGGSGGGSHHHHHHHHHH |
| Expression System | HEK293 |
| Molecular Weight | 72-85kDa |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. CHENGYING LIU, YAN HE, XIAO FENG, et al. Expression of EPHA5 in lung adenocarcinoma is associated with lymph node metastasis and EGFR mutation[J]. 2022,130(6):338-345. |
Background
EPHA5 is a member of the Eph family of tyrosine kinase receptors, which affect carcinogenesis. High expression of EPHA5 was found in NCI‐H460 and H1299 cells, while low expression was observed in A549 and SPC‐A1 cells. EPHA5 was knocked down in NCI‐H460 and H1299 lung cancer cell lines using siRNAs. EPHA5 receptor is differently expressed in lung adenocarcinoma tissues, in which positive and negative expression of EPHA5 was found in 58.1% and 41.9% of tissues, respectively.EPHA5 receptor plays a role in tumor promotion in lung adenocarcinoma and it is a potential target for lung adenocarcinoma treatment.
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