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Description
Mouse Breast Cancer Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4  & Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A added. |
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| Description | Composition:
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| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
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4.4 ★★★★★
Based on 23 reviews
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Product Reviews
★★★★★ 5
Perfect size
Color: Vanilla/Acorn
Perfect size . This will hold everything you need. It’s not too small not too big
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 4, 2026
★★★★★ 5
Colors and size
Color: Vanilla/Acorn
Perfect for my needs
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Reviewed in the United States on April 30, 2026
★★★★★ 5
Polished Everyday Crossbody, Though Priced Like a Designer-Branded Bag
Color: Vanilla/Acorn
This crossbody bag has a clean, classic look that works well for everyday use, errands, travel, or casual outfits where you want something more polished than a basic purse. The north-south shape gives it a slim profile, so it does not feel overly bulky, but it still has enough room for daily essentials like a phone, wallet, keys, small cosmetics, cards, and other small items.
The zipper closure is a practical feature because it keeps everything more secure than an open-top bag, especially when walking around, shopping, or traveling. The front and back zippered pockets are also useful for items you want quick access to, like a phone, receipt, lip balm, or transit card. I like that the layout keeps the bag organized without making it feel complicated.
The material is mostly PVC with some polyester and polyurethane, so it has more of a coated designer-bag feel rather than soft genuine leather. That makes it easier to wipe clean and more resistant to everyday marks, but buyers should know they are paying more for the brand/style than for leather construction. The bag feels structured and presentable, but it may not have the same long-term aging or softness as a real leather crossbody.
For comfort, the crossbody style is convenient because it keeps your hands free and distributes weight better than carrying a small handbag by hand. It is best for people who want a stylish everyday bag, not someone looking for a large tote or something that can carry bulky items.
For pricing, similar branded crossbody bags usually range from about $80–$200, depending on the brand, size, material, and whether it is leather or coated canvas/PVC. At around $180, this is on the premium side for a PVC-blend crossbody, but it is fairly typical for the Michael Kors price range. Overall, it feels worth considering if you like the style and brand name, but value-focused buyers may want to compare sale prices or color options before paying full price.
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Reviewed in the United States on May 14, 2026
★★★★★ 5
Stylish, Spacious, and Perfect for Everyday Use
Color: Brown/Acorn
absolutely love this Michael Kors Jet Set Travel crossbody bag! The design is great making it easy to pair with both casual and dressy outfits. It’s surprisingly spacious for its size and keeps all my essentials organized without feeling bulky. The quality of the materials and craftsmanship is excellent, and it feels very durable. This has quickly become my go to bag for everyday use and travel! Great price!
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Reviewed in the United States on April 28, 2026
★★★★★ 5
Designer quality and design
Color: Vanilla/Acorn
This is the second Michael Kors bag in my collection, and I’m pleased with both of them. These are quality items that are timeless and complement pretty much everything that i own.
At first, i thought that this was a travel bag in the sense that you would put toiletries in it, but it’s intended to be a smaller crossbody bag that you can travel with. It’s meant to hold essential items and look quite stylish while doing so.
I chose this color since my luggage set and toiletry bags are all cream and brown. Classic, chic, elegant and sophisticated without screaming that this is a designer bag.
This comes packaged beautifully and carefully so that it’s protected. The strap is adjustable on both sides. The bag lives up to Michael Kors quality and is worth every penny. I need a matching wallet or wristlet NOW.
Highly recommended if you appreciate designer products that have a higher price point but will last for years and always be in style.
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Reviewed in the United States on March 18, 2026
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