SKU: 40659614771

Alexa Fluor® 488 Mouse Anti-Rat CD45RA Antibody (S-R629)

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Description

Alexa Fluor® 488 Mouse Anti-Rat CD45RA Antibody (S-R629)Product Specification Host Mouse Antigen CD45RA Synonyms Leukocyte common antigen; high molecular weight form Location Cell membrane Accession 24699 Clone Number S R629 Antibody Type Mouse mAb Isotype IgG1,k Application FCM Reactivity Rt Positive Sample SD Rat splenocytes Purification Protein G Concentration 0. 2 mg ml Conjugation Alexa Fluor 488 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12 months

Product Specification


Host Mouse
Antigen CD45RA
Synonyms Leukocyte common antigen; high molecular weight form
Location Cell membrane
Accession 24699
Clone Number S-R629
Antibody Type Mouse mAb
Isotype IgG1,k
Application FCM
Reactivity Rt
Positive Sample SD Rat splenocytes
Purification Protein G
Concentration 0.2 mg/ml
Conjugation Alexa Fluor® 488
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 1.25μl per million cells in 100μl volume Rt

Background

CD45RA protein is a specific isoform of the CD45 receptor, also known as protein tyrosine phosphatase receptor type C (PTPRC), which is generated through alternative splicing of the CD45 gene. It is a marker for naive T cells and central memory T cells (TCM), characterized by the presence of exon 4 (A) but the absence of exons 5 (B) and 6 (C) in its mRNA. CD45RA is expressed on the surface of nucleated hematopoietic cells, including B cells, NK cells, naive/resting T cells, medullary thymocytes, and plasmacytoid dendritic cells. It plays a crucial role in the immune system by modulating T cell receptor (TCR) and B cell receptor (BCR) signaling pathways, facilitating the activation and proliferation of immune cells. In adults, CD45RA+ cells may help prevent autoimmune diseases, while reduced numbers of CD45RA+ CD4+ T cells have been associated with conditions such as biliary atresia, primary biliary cirrhosis, fetal distress, and preeclampsia. Pathologists use CD45RA to differentiate naive T cells (CD45RA+) from memory T cells (CD45RO+), although this distinction may not be applicable in adult allogeneic hematopoietic cell transplant recipients.

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SKU: 40659614771

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