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Description
Mouse PON2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Serum paraoxonase/arylesterase 2 (PON2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the Serum paraoxonase/arylesterase 2 (PON2) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Serum paraoxonase/arylesterase 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Paraoxonase-2 (PON2), also known as serum paraoxonase/lactonase 2, is an enzyme encoded by the PON2 gene. This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in tissues, is membrane-bound, and can act as a cellular antioxidant, protecting cells from oxidative stress. Its hydrolytic activity against acylhomoserine lactone, an important bacterial quorum sensing mediator, suggests that the encoded protein may also play a role in defense responses against pathogenic bacteria. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.7 ★★★★★
Based on 8 reviews
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Product Reviews
★★★★★ 5
A lightweight hose that performs like a heavyweight!
The Copper Head Expandable Garden Hose is my favorite hose to use when doing garden wastering chores. I am an avid gardener and need a sturdy hose that is portable. We have many gardens on our rural property, but no irrigation, so watering is essential in the summer droughts we’ve had lately. I like that the water drains out quickly and completely, which allows me to fill up containers until the hose is empty so we don’t waste the water. The outer cover is extremely durable, feels quite thick, so even if it catches on a branch, I don’t worry about the cover getting ruined. Also, the spray head and swivel connection make moving around while using the hose very easy. I can turn off the spray head with one finger and easily select another spray pattern as needed with the easy to use dial design. The hose does not tangle or kink, which also makes it easy to use. Even when the hose is filled with water, I am able to move around the gardens with ease. When I am done using the hose, I can put it in a reusable grocery bag to carry it in the garage and store it. I would not leave the hose full when done using it, nor leave it in the sun. When I am finished for the day, I drain it, put it in the bag, fold over the top, and leave it in the shade or put it in the garage so that it will not deteriorate. It’s my second year using it and it’s still like new. I love this hose! I recently bought two more 50 ft. ones!
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Reviewed in the United States on May 18, 2026
★★★★★ 5
BEST HOSE I EVER BOUGHT
I sure hope this thing lasts because it is simply the best garden hose I EVER bought! I’ve used it a lot in the 2 months since buying it, watering my plants, filling grow boxes, attaching to my pressure washer, etc. It does not kink, not even once.
The pocket pivot connector is a nice touch, and the spray nozzle works great without the usual dripping of cheaper models. The hose expands as soon as you turn it on, so be ready. I bought the 100’ and got a $30 discount at the time. $69 "all in" is still a lot of money for a garden hose, but I am super pleased thus far.
When you turn the water supply off, the hose will continue to spray for a couple minutes while it contracts, but again, it does so without tangles or kinks.
Super lightweight and very easy to handle with a strong water flow.
Only time will tell on durability, but thus far there’s been no “fraying” or signs of wear from dragging it across my driveway or rock landscape. I’ll re-evaluate after a season but I’m ready to replace my other hose with one of these.
For now, I love it and highly recommend.
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Reviewed in the United States on May 27, 2026
★★★★★ 4
I am hopeful this new version of Pocket Hose is better built than earlier versions!
I have not had great luck with earlier versions of Pocket Hose products! I have had leaks, hose blowout holes, leaks at the facet connector and sprayer difficulty! BUT, I wanted to give the new Copper Head Hose a try. I like all the new features and feel they have made good enhancements to the hose quality! I have had the new hose for a week or so and it appears to be of better quality than previous versions. The hose is working great and I am hopeful it will last longer than the last ones. However the product is expensive and this will be my last purchase of Pocket Hose if I have ANY issues in the future. I will update my review in the future!
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Reviewed in the United States on April 15, 2026
★★★★★ 5
love the pockets hose!!!
Style: Ballistic, Size: 75 FT
I usually don't do reviews but the pocket hose is so great I had to . It works so well, flexible , not heavy or cumbersome to move about as it curls back to water spigot when water turned off after using!!! Fun to watch I want to throw all my other hoses out !! I am elderly and this is so light and easy for me to use. I first saw as an add on TV and was skeptical! So happy I gave it a try!! We now have2 -5oft and 1 -75 ft.
Try it you'll like it !! I promise!!
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Reviewed in the United States on May 31, 2026
★★★★★ 5
Not as durable as advertised.
This hose is not as durable as advertised. First time I used it and didn't work. The hose itself was slit or had some other issue with the band inside because water leaked through the hose and did not reach the nozzle.
Update: I wrote to the manufacturer of the hose, Pocket Hose, regarding the defective hose. On May 30, 2026, they agreed to replace my hose under their warranty. On June 2, 2026, I received an email from them stating they recently updated their warranty policy to 30 days, and my notice was outside their 30-day warranty period. I then received an email from the seller of the hose, Bulb Head offering me a full refund on the hose. Something the manufacturer would not do. All I wanted was a replacement. I am changing my rating for the seller to 5 Stars for standing behind the sale of their product. Thank you, Bulb Head!
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Reviewed in the United States on May 30, 2026
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