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Description
Leptin Protein, HumanProduct Specification Species Human Synonyms LEPD, Obesity protein (OB), LEP Accession P41159 Amino Acid Sequence Val22 Cys167 Expression System E. coli Molecular Weight Approximately 16. 0 kDa, a single non glycosylated polypeptide chain containing 146 amino acids. Purity 97% by SDS PAGE or HPLC. Conjugation Unconjugated Tag No Tag Physical Appearance Lyophilized Powder Storage Buffer 50 mM PB, with 0. 02% (v v) Tween 20, pH 3. 5. Reconstitution
Product Specification
| Species | Human |
| Synonyms | LEPD, Obesity protein (OB), LEP |
| Accession | P41159 |
| Amino Acid Sequence | Val22-Cys167 |
| Expression System | E.coli |
| Molecular Weight | Approximately 16.0 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids. |
| Purity | ＞97% by SDS-PAGE or HPLC. |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 50 mM PB, with 0.02% (v/v) Tween-20, pH 3.5. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage | For long term storage, the product should be stored ≤ -20℃. |
Background
Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. The absence of leptin (or its receptor) leads to uncontrolled food intake and resulting obesity. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models. Recombinant Human Leptin is a 16.0kDa protein containing 146 amino acid residues.
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