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Description
Human TNFRSF17 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. 3. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. 4. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a capture antibody against Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | ELISA Kit for Tumor Necrosis Factor Receptor Superfamily, Member 17 | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | The protein encoded by this gene is a member of the TNF receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes and may be important for B cell development and autoimmune responses. This receptor has been shown to specifically bind to tumor necrosis factor (ligand) superfamily member 13b (TNFSF13B/TALL-1/BAFF), leading to activation of NF-kappaB and MAPK8/JNK. This receptor also binds to various TRAF family members, thereby transducing signals for cell survival and proliferation. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.6 ★★★★★
Based on 29 reviews
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Product Reviews
★★★★★ 4
Matcha
Flavor Name: Culinary, Size: 1.06 oz (Pack of 1)
I haven’t decided if I really like Matcha, as I got this, just to find that out. I find that the packaging is interesting, and it is best, probably, to store it in the package it comes in. It has a mild flavor, and I am just making tea with it, as I don’t bake any more. It is a bright green powder with a smooth, mild taste, and in a clever package.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 7, 2026
★★★★★ 5
A Premium Matcha Experience for the True Connoisseur
Flavor Name: Culinary, Size: 1.06 oz (Pack of 1)
I recently had the pleasure of trying the Jade Leaf Matcha Organic Premium Ceremonial Grade Matcha Green Tea Powder, and it truly lives up to its reputation as a high-quality product. As someone who enjoys matcha regularly, I was eager to compare this offering to others on the market, and I have to say I was very impressed.
The first thing I noticed was the vibrant green color of the powder. It’s a rich, deep green that is indicative of the quality and freshness of the leaves. When prepared, the tea has a smooth, silky texture and a natural sweetness that makes it enjoyable both on its own and as an addition to smoothies or lattes. I appreciate that this matcha doesn’t have the bitterness that can sometimes accompany lesser-quality varieties, making it a perfect choice for ceremonial use or a relaxing daily treat.
As a ceremonial grade matcha, it’s clear that Jade Leaf has carefully sourced and processed the tea leaves. The flavor is balanced and complex, with grassy, vegetal notes followed by a subtle sweetness. It has the perfect level of umami, which is an essential characteristic of a high-quality matcha. I’ve tried a variety of brands, but this one stands out for its consistency and superior taste.
The packaging is also noteworthy. The resealable bag ensures the matcha stays fresh, which is crucial for preserving its delicate flavor and antioxidants. It’s easy to scoop out the right amount without making a mess, and the instructions on the back are clear and helpful for those new to preparing matcha.
Whether you’re a seasoned matcha drinker or new to the world of green tea, this Jade Leaf matcha provides a top-notch experience. I highly recommend it for anyone looking for an authentic, high-quality matcha that delivers excellent taste and a sense of calm and clarity with every sip.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 12, 2026
★★★★★ 5
The all day energy
Flavor Name: Culinary, Size: 1.06 oz (Pack of 1)
Great taste when you know how to make it..gives me full of energy, the smell.is fresh, the color is the perfect green, not grit and the price is reasonable. Drink it in a tea drink or smoothie . Smooth texture. Very drinkable.
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Reviewed in the United States on May 26, 2026
★★★★★ 3
Not an affordable option for organic matcha
Flavor Name: Culinary, Size: 1.06 oz (Pack of 1)
Great product however very misleading on the serving amount per package. For a full flavor robust cup of matcha from this product you will get an average of 15 servings. There are much better affordable options available.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 12, 2026
★★★★★ 5
It has no sugar
Flavor Name: Culinary, Size: 1.06 oz (Pack of 1)
Very matcha taste and texture. I used this to create a matcha butter cream for my enchanted garden themed cupcakes and it was so delicious. There was no spiciness and I was able to use the rest to make matcha for the kids so I definitely got a good value for my money. I did not have any digestive problems using this. As I did mix it with ripple non dairy milk
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 3, 2026
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