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Description
PE Rabbit Anti-FMC63 Antibody (S-2163-77)Product Specification Host Rabbit Antigen FMC63 scFv Immunogen Recombinant Protein Clone Number S 2163 77 Antibody Type Recombinant mAb Isotype IgG Application FCM Purification Protein A Concentration 0. 2 mg ml Conjugation PE Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 2 to 8 C as supplied Dilution application dilution species FCM 1. 25l per million cells
Product Specification
| Host | Rabbit |
| Antigen | FMC63 scFv |
| Immunogen | Recombinant Protein |
| Clone Number | S-2163-77 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | PE |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Species independent |
Background
FMC63 scFv (single-chain variable fragment) is a recombinant protein derived from the variable regions (VH and VL) of the anti-CD19 monoclonal antibody FMC63, linked by a flexible peptide spacer (e.g., (Gly₄Ser)₃). It specifically binds to the B-cell surface antigen CD19 with high affinity (KD ~1-10 nM) and is a key targeting component in FDA-approved CAR-T therapies, such as Kymriah®. The humanized FMC63 scFv retains potent antigen recognition while minimizing immunogenicity, making it highly effective in treating B-cell malignancies, including acute lymphoblastic leukemia (ALL) and non-Hodgkin lymphoma (NHL). Ongoing research focuses on optimizing its affinity, stability, and functionality to enhance the safety and efficacy of CAR-T cell therapies.
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