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Description
Mouse XBP1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an X-Box Binding Protein 1 (XBP1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of X-Box Binding Protein 1 (XBP1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse X-Box Binding Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | X-box binding protein 1, also known as XBP1 or TREB5, is a protein encoded by the XBP1 gene. The XBP1 gene is located on chromosome 22, while a closely related pseudogene has been identified and mapped to chromosome 5. This protein is a transcription factor that regulates the expression of genes important for the normal functioning of the immune system and cellular stress responses. It is a bZIP domain-containing transcription factor. It was first identified by its ability to bind to Xbox, a conserved transcriptional element in the human leukocyte antigen (HLA) DRα promoter. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.5 ★★★★★
Based on 7 reviews
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Product Reviews
★★★★★ 5
Quite literally my perfect water bottle
Color: Black Cherry, Size: 40 Ounces
This water bottle is perfect for me and is absolutely everything I wanted in a water bottle. Easy to clean, not too heavy and the handle makes it easy to carry around anyway, keeps water cold for a day but not much longer than that (but that's okay). I haven't dropped it yet so I don't know how well it'll hold up when I inevitably do but it seems plenty sturdy to handle it. I'm so excited about the color too because it's my favorite color and from what I can tell the dark cherry is an Amazon exclusive so I'm glad I found this. I love that it's still slender and can fit in standard cup holders while being a bigger bottle holding 40oz, which was my biggest priority.
I've never had an Owala before so I wasn't sure what I'd think of the freesip mouth but I love it. I don't usually need to chug but having the option is nice too! If I ever need to replace this beauty I'll definitely get another Owala.
I really could not ask for a more perfect water bottle. I don't even usually write reviews but I love this thing so much I just had to. I usually wouldn't spend this much on a water bottle but given all the praise I have for it the price is perfectly reasonable to me (and I try to be smart with money and responsibly pick when to spend more on something or be more frugal.) This is definitely worth the higher price than I have spent in the past on water bottles, and I'm pretty confident it'll last me a long time anyway, and I use it every single day too.
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Reviewed in the United States on February 26, 2026
★★★★★ 5
Best Water Bottle Ever!!
Color: Strawberry Fields, Size: 40 Ounces, Color: Strawberry Fields, Size: 40 Ounces
This is the greatest water bottle ever! I ended up buying 2 more after this. First, it's 40oz in a very easy to hold cup, that fits into car cup holders. It's leak proof which is amazing because every other bottle I've tried leaks in some way. You have 2 ways of drinking. You have the straw, and also the sip option. The handle is strong and sturdy. It locks to make sure it won't open on you on accident. Overall this is the greatest bottle I've ever had, and I'd highly recommend to anyone looking for a great bottle!
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Reviewed in the United States on May 2, 2026
★★★★★ 4
Doesn’t leak, durable, keeps drinks cold
Color: Sparkling Sherbet, Size: 40 Ounces
Absolutely love this water bottle. I am a water bottle junkie and this one is my favorite. It doesn’t leak and the fact that I can guzzle or sip through a straw is a major plus. It is kind of heavy but it is a big thermos. The only reason I didn’t give five stars is because the bottom doesn’t fit in all cup holder. It keeps my drinks cold all day and is super easy to clean. It is very sturdy and well made. I figured after using for a while, the lid wouldn’t pop open as easy as it did when it was new, but it still does. I also love the lock feature incase the button accidentally gets pressed when I am carrying it around in my bag.
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Reviewed in the United States on April 11, 2026
★★★★★ 5
Compact, incredibly absorbent, and a travel essential.
Color: Gray, Size: 48.00" x 24.00"
The Rainleaf microfiber towel has been a fantastic addition to my gear. It’s remarkably compact and lightweight, which makes it perfect for tossing into a gym bag or packing for trips without taking up unnecessary space. Despite its size, it’s surprisingly super absorbent and dries much faster than a standard cotton towel, which is a huge plus when you're on the move. It’s well-made, feels durable, and is definitely a must-have for anyone who travels, camps, or hits the gym regularly.
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Reviewed in the United States on May 22, 2026
★★★★★ 5
Great Travel Towel !!
Color: Mint Color, Size: 72.00" x 40.00"
I travel for work. The last few years I've noticed the quality of hotel towels has gone downhill. I suspect that they are not replacing them as often to save money. So not only are hotel towels too small, they are thread bare, thin and scratchy. So I thought about bringing my own towel, but, it's not practical as they are heavy and bulky to pack, and they take a long time to dry. This Rainleaf Microfiber towel is the answer to my prayers. I bought the big one, 70 X 40. I tested it at home. It is kinda strange to dry off with it because it's different. Not bad, just different. It smooth but not soft and fuzzy like a cotton towel. BUT, it dries amazingly well. It doesn't even get that wet. It's more like patting dry then rubbing you dry, it you know what I mean. Once you get used to it, it works great. Even though is it huge at 70" X 40", it weighs less than 1 pound. Super small and great for packing. Corner pocket would be good at the beach for keeping valuables out of sight. It's my new go-to hotel towel. I may even bring it on my cruise !
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Reviewed in the United States on April 27, 2026
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