SKU: 48583938464

Alexa Fluor® 647 Rat Anti-Mouse CD197 (CCR7) Antibody (S-R528)

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Description

Alexa Fluor® 647 Rat Anti-Mouse CD197 (CCR7) Antibody (S-R528)Product Specification Host Rat Antigen CD197 (CCR7) Synonyms C C chemokine receptor type 7; C C CKR 7; CC CKR 7; CCR 7; Epstein Barr virus induced G protein coupled receptor 1 (EBI1; EBV induced G protein coupled receptor 1); MIP 3 beta receptor; Cmkbr7; Ebi1; Ebi1h; Ccr7 Location Cell membrane Accession P47774 Clone Number S R528 Antibody Type Rat mAb Isotype IgG2a,k Application FCM Reactivity Ms Positive Sample BALB c mouse splenocytes Purification

Product Specification


Host Rat
Antigen CD197 (CCR7)
Synonyms C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR-7; Epstein-Barr virus-induced G-protein coupled receptor 1 (EBI1; EBV-induced G-protein coupled receptor 1); MIP-3 beta receptor; Cmkbr7; Ebi1; Ebi1h; Ccr7
Location Cell membrane
Accession P47774
Clone Number S-R528
Antibody Type Rat mAb
Isotype IgG2a,k
Application FCM
Reactivity Ms
Positive Sample BALB/c mouse splenocytes
Purification Protein A
Concentration 0.2 mg/ml
Conjugation Alexa Fluor® 647
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 1.25μl per million cells in 100μl volume Ms

Background

CD197, also known as CCR7, is a protein encoded by the CCR7 gene in humans and belongs to the G protein-coupled receptor family. It is a transmembrane protein with seven transmembrane domains, which is coupled with heterotrimeric G proteins to transduce signals downstream through various signaling cascades. CCR7 binds to two ligands: the chemokines CCL19 and CCL21. The main function of CCR7 is to guide immune cells to immune organs such as lymph nodes, thymus, and spleen by detecting specific chemokines secreted by these tissues. It is expressed on adaptive immune cell types, including thymocytes, naive T and B cells, regulatory T cells, central memory lymphocytes, and dendritic cells. CCR7 plays a crucial role in the maturation of dendritic cells and the trafficking of T cells within secondary lymphoid organs.

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SKU: 48583938464

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4.7 ★★★★★
Based on 30 reviews
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Verified Purchase
William L. Pogue
New York, US
★★★★★ 5
Five Stars
Format: Paperback
good job
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 5, 2018
M
Michael Griswold
Natrona Heights, US
★★★★★ 4
A Book For Audio
Format: Audiobook
The Art of Storytelling from Parents to Professionals is the first book that I can be confident in saying is better as an audio version than it would be in a paper or Kindle form because you can here the verbal inflections and the storytellers can change character, voice much easier than the printed word might. It also captures the listeners attention as the author herself can connect in a lot more personal and intimate way. My concern is while I can understand what the author is getting at, I am not aspiring to be an oral performance style storyteller and there was not enough of a reach out from the world of oral storytelling to the written story. I mean how many of us are going to get up on stage and tell stories? I guess you can take the skills from one realm and use them elsewhere, but the connection may not be made so easily. This was an audiobook that I had a lot of fun with, even if I didn’t quite get what I was hoping for from it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 10, 2020
L
Louis LaSalle
Cuba, US
★★★★★ 5
Great Overview of the Art of Storytelling
Format: Audiobook
I chanced on this as an Audible "freebie" to keep on the list for when I was out of credits. Well, it's excellent, and well worth the listen. And excellent survey of the topic spanning topics of performance (preparing, voice, body language, projection), various aspects of framing (culture, age, ethnicity, audience size), story structure and so on This point is for Hannah B. Harvey, if perchance she reads tese reviews. One point of modern storytelling and writing that is not brought out in your lectures, is that some of the best villain/antagonists are actually the heroes/protagonists of their own stories. This is tangentially alluded to in talking about story viewpoints, but not to the extent that it can be an entirely new story, as Wicked and Maleificent turned The Wizard of Oz and Sleeping Beauty on their heads. And even in the 1960's, many a Bond 007 villain was trying to create what they imagined to be a better world. It's useful to consider in storytelling, as far too many people have forgotten/fail to see the fundamental moral ambiguities of life, and I suspect that goes a long way to explaining the extreme partisanship we see in the world today.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 17, 2023
D
Doodlebugs
Pawtucket, US
★★★★★ 3
Sadly, I found the tips and the examples in this lecture to be very simplistic and uninspiring.
Format: Audiobook
I expected a professional storyteller to be able to keep my interest but I found the presentation to be quite boring. I got nothing out of it that I didn’t already know from just being an avid reader. It felt like a high school lecture. Sigh!!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 15, 2019
E
Emilia Taneva
Birmingham, US
★★★★★ 5
Great course on how to improve your storytelling abilities
Format: Audiobook
Storytelling is a great way to get a point across, and most of us digest information through stories. But when it comes time to tell a story, most of us don't have the skills to make the story engaging or impactful. That's where this course on storytelling comes in. You'll get good pointers on vocabulary, body language, tone, along with deeper pointers about how to shape the view of the story and how to refine its characters. It's all very detailed but it's all very interesting and well worth it. This course will help you see how useful storytelling can be in your personal and professional life, especially if you're in a field where you have to do a lot of public speaking.
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Reviewed in the United States on August 21, 2021

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