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Description
Human Killer cell lectin-like receptor subfamily G member 1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Killer cell lectin-like receptor subfamily G member 1. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the presence of Killer cell lectin-like receptor subfamily G member 1 in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Killer cell lectin-like receptor subfamily G member 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Killer cell lectin-like receptor subfamily G member 1 is a protein encoded by the KLRG1 gene. KLRG1 is a lymphocyte co-inhibitory or immune checkpoint receptor primarily expressed on late-differentiated effector and effector memory CD8+ T and NK cells. Its ligands, with similar affinities, are E-cadherin and N-cadherin, markers of epithelial and mesenchymal cells, respectively. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
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4.2 ★★★★★
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Product Reviews
★★★★★ 5
Beautiful quality made tv stand.
Color: Walnut
Beautiful TV stand. Easy to assemble. I took a chance on this stand matching the new end tables I got from a different store and manufacturer. They match perfectly. I couldn't be happier with the color of the wood, the quality construction and minimalist clean look. It looks great in my living room.
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Reviewed in the United States on March 9, 2026
★★★★★ 5
Modern and sleek
Item Package Quantity: 1
I haven't assembled this yet but I'm really looking forward to using it as a nightstand - the style looks really sleek and modern and it seems like it'll fit perfectly in a small space.
It's worth noting that it's made of particle board rather than solid wood, which is pretty standard at this price point, so just go in with that expectation. The design looks really clean and the storage shelf underneath is a practical bonus.
Hoping it holds up well over time but based on first impressions and the overall look, I think it's going to be a really nice and affordable addition to the bedroom!
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Reviewed in the United States on May 22, 2026
★★★★★ 3
Decent looking but not very sturdy
Item Package Quantity: 1, Item Package Quantity: 1
This table looks pretty nice once it is fully assembled and I appreciated that all the parts were clearly labeled. There were no missing parts, and in fact there were some extras. That made the instructions easier to follow and helped speed things up. From unpacking everything to finishing assembly it took me around 30 minutes.
There are a few quality issues though. The black finish feels very thin and started chipping around the screws while I was tightening them down. It does not seem like it will hold up very well over time if it already shows wear during assembly.
The biggest issue is the stability. Even after tightening everything, the table still feels a little shaky. The set screws near the bottom base pieces do not stay tight for very long and kept loosening while I was putting it together. I assembled it by myself and the legs kept shifting and falling over because those connections would not stay secure. This is definitely easier if you have another person helping.
The packaging also could have been better. The box had a lot of particle board dust and debris inside, so unpacking everything was a bit messy.
The item description says it can hold up to 66 pounds, but after putting it together I personally would not trust it with anywhere near that much weight. I originally planned to use it for a small aquarium that weighs around 20 pounds, and even that made me uncomfortable once I saw how flimsy the table felt assembled. I think it is better suited for lightweight items and I'd only store something like a couple of books or a lightweight lamp or a couple of picture frames.
Overall it is not terrible and it does look reasonably attractive, but the build quality does not really match the current fifty dollar price in my opinion. It should be priced lower. It works for light decorative use, but I would not expect anything especially sturdy or long lasting.
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Reviewed in the United States on May 26, 2026
★★★★★ 5
Solid table
Item Package Quantity: 1
Good size! Would be easier with 2 people putting in together- if you follow directions! Cute table!
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Reviewed in the United States on May 24, 2026
★★★★★ 5
Great end table
Item Package Quantity: 1
This is always a great brand. Easy to assemble. Strong and durable. Looks amazing. As expected and at an amazing price.
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Reviewed in the United States on May 30, 2026