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Description
Human CCR7 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Chemokine CC-Motif Receptor 7 (CCR7) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Chemokine CC-Motif Receptor 7 (CCR7) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Chemokine C-C-Motif Receptor 7 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Chemokine CC-motif receptor 7 (CCR7) is a protein encoded by the CCR7 gene. Two ligands for this receptor have been identified: chemokine (CC motif) ligand 19 (CCL19/ELC) and (CC motif) ligand 21 (CCL21). It has also recently been designated CD197 (cluster of differentiation 197). The protein encoded by this gene is a member of the G protein-coupled receptor family. This receptor has been identified as a gene induced by Epstein-Barr virus (EBV) and is thought to mediate the effects of EBV on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates both B and T lymphocytes. It has been shown to stimulate the maturation of dendritic cells. It is also involved in T cell homing to various secondary lymphoid organs, such as lymph nodes and spleen, and in T cell trafficking within the spleen. Activation of dendritic cells in peripheral tissues induces cell surface expression of CCR7, which recognizes CCL19 and CCL21 produced in lymph nodes and increases dendritic cell expression of costimulatory molecules (B7) and MHC class I or MHC class II. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.2 ★★★★★
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Product Reviews
★★★★★ 5
Light enough for one person to move, sturdy and so versatile
Size: 48" X 10', Color: Grey, Number of Items: 1
The AmazingSpark Soundproof room divider panel has been a fantastic addition to my classroom. Its sound-absorbing design noticeably reduces noise, which makes small group instruction, testing sessions, and independent work time much more manageable. The panel is lightweight yet sturdy. The included stand and straps keep it securely in place. I appreciate how easily it folds - setup and storage take only a minute, which is perfect for transitioning from one activity to another. The grey color looks clean and professional, and I can use push-pins to hang displays on the board. The divider delivers exactly what teachers need: privacy, noise reduction, flexibility, and durability. The noise reduction feature has been beneficial in my classroom when students are recording projects. While the class is working quietly on other activities, a small group can move to the opposite side of the panel and record their audio files. When we listened to the files, we could not hear any background noises, with the exception of the noises they wanted in their recording. This panel is made to last, and I would recommend it for anyone who is looking for an acoustic panel that is flexible. The panel is extremely versatile, and because of that, I think it is a good value for the money. Noise reduction, privacy screen, or to section off one area from the rest of the room are just a few of the uses of this product.
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Reviewed in the United States on November 24, 2025
★★★★★ 4
Easy To Use
Size: 48" X 6', Color: Grey, Number of Items: 1
Panel measures 48x6 and is super easy to install/put up. It's made of a soft thick material that has an accordion shape style/design and stands up nicely wherever you put it. Quality of the material is good- it's light, easy to use and can be moved with ease. It's also sturdy for being soft and does the job if you're looking for a room divider if this size. The height is shorter than the standard style divider but serves the purpose. Says it has 'soundproof properties'- may absorb some noise but you can still hear. I'd recommend it if you're looking for this style of partition wall for privacy. Noise factor not sure but the material it's made of looks like it should absorb some noise. Moreso recommended for the function, separation and quality.
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Reviewed in the United States on February 12, 2026
★★★★★ 5
A Game-Changer for Focus and Privacy!
Size: 48" X 6', Color: Grey, Number of Items: 1
I was excited to try out this Soundproof Room Divider Panel, and it has genuinely exceeded my expectations! I set up the 48" x 6' grey panel in my home office, which is often subject to noise from the rest of the house, and the difference in my ability to concentrate is remarkable. The combination of the acoustic foam and felt really does a fantastic job of dampening ambient sounds, making it a true soundproofing and privacy solution. Assembly was a breeze; the included curved support stands attach quickly, and the panel is stable yet lightweight, making it super easy to move around and reposition as needed. The fact that it folds down to a compact 48" x 15" is a huge bonus for storage, but I honestly haven't needed to put it away because it's so useful!
I love the multi-functional nature of this partition. Beyond just reducing noise, the felt surface acts as an excellent display board, I've already started using thumbtacks to pin up project notes and schedules. It’s given me a dedicated, quiet, and visually organized workspace. The flexibility of the foldable design means I can position it in various shapes to customize the privacy zone. For anyone who needs to carve out a peaceful space for studying, working, or even just relaxing in a shared environment, this is an excellent, high-quality solution. The grey color is sleek and unobtrusive, blending seamlessly into my decor. I highly recommend this portable acoustic divider—it's been a great addition to my home.
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Reviewed in the United States on October 16, 2025
★★★★★ 4
Not sure about the price, but solid!
Size: 48" X 10', Color: Grey, Number of Items: 1
This soundproof divider worked surprisingly well for creating a quieter workspace. It doesn’t block all noise, but it definitely reduces echoes and softens background sounds. The panel is sturdy, easy to fold, and the stand keeps it stable. Great for extra privacy or separating areas without drilling anything.
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Reviewed in the United States on November 24, 2025
★★★★★ 5
A Lifesaver for Peace and Quiet in a Noisy Apartment
Size: 48" X 10', Color: Grey, Number of Items: 1, Size: 48" X 10', Color: Grey, Number of Items: 1
Living with a very loud senior with hearing issues can be quite challenging, but this soundproofing room divider has been a game changer! The panel, crafted with acoustic foam and felt, significantly reduces noise, creating a peaceful and private environment in my home. It's perfect for setting up in my study room or bedroom, allowing me to focus without distractions.
This divider isn't just for noise reduction; it also serves as a handy display board. I can easily use thumbtacks and hook-and-loop fasteners on its felt surface for presentations or home education purposes. The versatility makes it ideal for office meetings and visual displays too.
The package includes a foldable privacy panel, two curved support stands, a fastening strap, and non-slip tape. Measuring 48" X 6' when open, it folds down to a compact 48" x 15", providing flexible layout options. Assembly is a breeze—just attach the curved legs, and it's ready to go!
I appreciate that the divider is lightweight and portable, making it easy to move around. The design is safe, with no sharp edges, and includes anti-slip tape and stable stands to ensure it stays in place. Overall, this soundproofing divider has truly improved my living situation, and I highly recommend it to anyone needing some peace and quiet in a noisy environment!
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Reviewed in the United States on October 13, 2025
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