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Description
Human MMP-7 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a matrix metalloproteinase 7 (MMP-7) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of matrix metalloproteinase 7 (MMP-7) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Matrix Metalloproteinase 7 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Matrix metalloproteinase-7 (MMP-7) is an enzyme encoded by the MMP7 gene. It is also known as matrin, matrix metalloproteinase pump 1, PUMP-1 protease, PUMP, metalloproteinase pump-1, and putative metalloproteinase. Human MMP-7 has a molecular weight of approximately 30 kDa. The promoter region of the human MMP7 gene contains two or more sites homologous to NR-IL6 binding sequences, suggesting that MMP7 can bind to both IL-1 and IL-6. Furthermore, MMP7 mRNA levels are elevated in human mesangial cells following treatment with tumor necrosis factor α (TNF-α) and IL-1β. It is normally expressed in epithelial cells, including the ductal epithelium of exocrine glands of the skin, salivary glands, pancreas, intestinal and reproductive glands, liver, and breast. Furthermore, MMP7 is highly expressed on the luminal surface of dysplastic glands in human colorectal cancer. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.7 ★★★★★
Based on 16 reviews
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Product Reviews
★★★★★ 5
Recommend. Great for Home office
Color: White, Number of Items: 1, Color: White, Number of Items: 1
I am very pleased with this chair. I’m working on building my home office and it’s important to me and have a look that is comforting and clean looking the chair was easy to put together. Didn’t take very long at all. It’s sturdy. It rises up and down to fit your height and it’s comfortable. It was a great purchase. I’m glad I got it.
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Reviewed in the United States on January 28, 2026
★★★★★ 1
Seller Stall Tactics
Color: White, Number of Items: 1, Color: White, Number of Items: 1
My chair arrived last Wednesday, but I only assembled it today. It looks overwhelming as you take all of the pieces out of the box, but it is straightforward to assemble. Took me less than 45 minutes start to finish. Assembled, the chair is really nice. Many reviews talk about the incessant squeaking. I'm guessing it has to do with how they assembled the chair. Mine does not make a sound. The seat is very firm, it may be uncomfortable for some people; I'll have a better idea after I use it for awhile. I gave it 4 stars because the plastic lever to raise/lower the chair, the underside broke off in 3 pieces! Fortunately I was able to fit the pieces back, and used liquid Gorilla Glue all over the broken seams. So far it's holding up well. Visually the chair is beautiful.
UPDATED 6/1/20 - I originally gave this chair 4 stars. As you can see in my original post, I took one star off for a crucial piece that was broken inside the box. I read reviews where customers who experienced issues were not getting assistance from the seller. I am now in that same issue. After I initially e-mailed the seller regarding a replacement part for that one piece, they are repeatedly e-mailing me asking me is this part broken, is that part also broken. My last e-mail to them stated that I have explained the issue in detail multiple times and if I am sent one more e-mail asking if another part is broken, I will know this is a stall tactic. I have an e-mail this morning asking if the gas lift is broken. I've contacted Amazon to contact the seller with the understanding that if they stall Amazon as well, a request will be made for a full refund. I am NOT the one to play with when it comes to shady customer service. If I could give negative stars based on the seller alone, I would.
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Reviewed in the United States on May 27, 2020
★★★★★ 5
Well made value chair
Color: Black, Color: Black
Great value and well constructed. Easy to assemble. It’s very light and maneuverable. It’s reasonably comfortable for occasional use although I would prefer something with more support if I were using this every day. For my needs, however, it’s perfect. Adjustments are easy – especially height, changing from tilting to rigid. I also appreciate the swing up arms, which was a feature that I particularly liked with this chair.
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Reviewed in the United States on March 19, 2026
★★★★★ 5
Comfortable and Affordable
Color: Black
I normally dislike chairs like this, but with the budget I had I had to go with something more affordable. It is perfect and love how the handles go up to make a little bit more flush with the desk giving me some more room. The cushion is amazing, lumbar support is perfect for me. Very stable and I sometimes almost catch myself reclining back and wanting to fall asleep. Quality sturdy materials make it easy to attach any additional neck accessories to the chair. 10/10 would recommend.
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Reviewed in the United States on April 2, 2026
★★★★★ 4
Comfortable and Stylish Office Chair
Color: Black
The NEO Chair Office Desk Chair Computer High Back is a great addition to my workspace. It was easy to assemble and feels sturdy once put together. The high back provides good support, especially during long hours at my desk. The cushioning is comfortable, and the chair rolls smoothly without any issues. Overall, it looks professional and offers solid comfort for the price.
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Reviewed in the United States on February 28, 2026