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Description
UniOne® TR-FRET Human BCL-XL/VHL PROTAC Binding KitProduct Specification Host Human Stability & Storage 80 Background This kit uses HomogeneousTime Resolved Fluorescence (TR FRET) technology to measure the molecular glueBCL XL DEGRADER 2 and test compound mediated interaction between VHL andBCL XL. This method enables a simple and rapid high throughput format for screeningsmall molecules that can mediate the interaction between VHL and BCL XL. As shown, the interaction betweenVHL and BCL XL is
Product Specification
| Host | Human |
| Stability & Storage | -80℃ |
Background
This kit uses HomogeneousTime-Resolved Fluorescence (TR-FRET) technology to measure the molecular glueBCL-XL DEGRADER-2 and test compound-mediated interaction between VHL andBCL-XL. This method enables a simple and rapid high-throughput format for screeningsmall molecules that can mediate the interaction between VHL and BCL-XL.
As shown, the interaction betweenVHL and BCL-XL is detected using an Eu-labeled anti-Tag1 antibody (TR-FRETdonor) and an Acceptor-labeled anti-Tag2 antibody (TR-FRET acceptor). Since themolecular glue BCL-XL DEGRADER-2 mediates the interaction between VHL andBCL-XL, the donor and acceptor antibodies are brought into proximity.Excitation of the donor antibody triggers Fluorescence Resonance EnergyTransfer (FRET) to the acceptor antibody, resulting in a specific emissionsignal at 665 nm from the acceptor. This specific signal is proportional to thedegree of interaction mediated by BCL-XL DEGRADER-2 between VHL and BCL-XL.This homogeneous assay is simple to perform and requires no washing steps.
Components
Table 1. Reagents and Storage Conditions
Component |
Concentration |
100TESTS |
500TESTS |
2500TESTS |
10000TESTS |
Storage Temp. |
|---|---|---|---|---|---|---|
Tag1-VHL protein |
50× |
10μL |
40μL |
200μL |
800μL |
-80°C |
Tag2- BCL-XL protein |
50× |
10μL |
40μL |
200μL |
800μL |
-80°C |
BCL-XL DEGRADER-2 |
0.3 mM |
5μL |
20μL |
50μL |
200μL |
-80°C |
Anti-Tag1 Eu dye antibody |
50× |
10μL |
50μL |
250μL |
1000μL |
-80°C |
Anti-Tag2 AC antibody |
100× |
5μL |
25μL |
125μL |
500μL |
-80°C |
Detection buffer |
10× |
400μL |
2 mL |
10 mL |
40 mL |
-80°C |
*Reagents should be avoided from repeated freeze-thaw cycles. Aliquot upon first thaw and store under recommended conditions. Valid for one year.
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Exchange/Return Notes
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