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Description
Human HGFR ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minutes, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Sensitivity | 0.12 ng/mL | |||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a Hepatocyte Growth Factor Receptor (HGFR) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Hepatocyte Growth Factor Receptor (HGFR) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Hepatocyte Growth Factor Receptor ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | The hepatocyte growth factor receptor (HGFR), also known as the tyrosine protein kinase Met or c-Met, is a protein encoded by the MET gene in humans. This protein possesses tyrosine kinase activity. The primary single-chain precursor protein undergoes posttranslational cleavage to produce α and β subunits, which are disulfide-linked to form the mature receptor. MET is a single-pass tyrosine kinase receptor that is essential for embryonic development, organogenesis, and wound healing. Hepatocyte growth factor/scatter factor (HGF/SF) and its splice isoforms (NK1 and NK2) are the only known ligands for the MET receptor. It is normally expressed by epithelial cells, while HGF/SF expression is restricted to mesenchymal cells. When HGF/SF binds to its cognate receptor, MET, it induces its dimerization through a mechanism that is not yet fully understood, leading to its activation. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.4 ★★★★★
Based on 6 reviews
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Product Reviews
★★★★★ 1
Very glitchy mouse won't accept clicks, made me reinstall windows
Color: White
TLDR: Ask ChatGPT about this mouse and it'll tell you that other users have reported issues with this mouse not clicking properly. It will tell you that this is a known issue with this mouse.
So I'm an IT expert (programmer, been using PC's since Windows 3.1). And I could not get this mouse to work right. The mouse doesn't work right when the computer goes to sleep, and then wakes from sleep. This problem occurs on Windows 11 pro.
When the mouse is connected in Bluetooth mode: First problem is, the mouse will NOT reconnect to the PC when the PC wakes from sleep, unless you have "Show notifications to connect using Swift Pair" disabled in windows Bluetooth settings. But setting is on by default. Second problem is, even with this setting disabled, and even if the mouse successfully reconnects when the computer wakes up, the computer will NOT accept clicks when it wakes up. This mouse apparently drops a phantom click that it never releases. The only way I could find to get rid of that phantom click is by pressing CTRL+ALT+DEL every time the computer wakes. This resets the phantom click and the mouse would function normally. But it's super annoying to have to press CTRL+ALT+DEL every time the PC wakes, especially since this mouse is supposed to be a gift to my little girl, I don't want her to have to deal with it not working perfectly. Also the mouse would sometimes stop accepting clicks even in the middle of playing games, but this only happened rarely in Bluetooth mode.
On the wireless USB mode: The mouse cursor is NOT visible when windows first boots. You can't use the mouse to get to the box to type your PIN number to unlock the computer. You must use the keyword to get into windows, and then the cursor appears. This problem doesn't happen when using the mouse in Bluetooth mode, only in the wireless USB mode. And then the phantom click issue is MUCH more frequent in the wireless USB mode, and doesn't require the computer to wake from sleep, but happens randomly even in the middle of playing games... suddenly you can't click anything. And when waking from sleep, or when the mouse "goes to sleep", when it wakes up there's issues with clicks... and sometimes the cursor isn't even visible.
Connecting a second mouse: When the computer stops accepting clicks, then even the second mouse would not be able to click. All connected mouses would stop being able to click. But this problem of not being able to click, would ONLY occur if the Razor Orochi V2 mouse was connected to the computer either via Bluetooth or wireless USB. If I disconnected the Orochi V2 mouse, then the problem never happens. So it's definitely this mouses's fault.
But initially I didn't realize this mouse was the culprit. Because it affected all connected mice. So I thought, at first, that it was a windows issue... maybe a bad driver I had installed in the past. So I ended up reinstalling windows, trying to fix any potential driver issues. It wasn't until after that, when I discovered that the problem was this mouse all along. I was super upset to have reinstalled windows, and lost all my software and settings, only to then discover that the problem was still there and it was this mouse all along.
As for getting my money back, amazon did not offer me an option to return the mouse and get a replacement. It only lets me send it back for a full refund. No option for replacement. So I think I bought this mouse when it was on sale. So it seems like they are expecting me to send it back, and then repurchase it at the new higher price, if I want to try it again. It's possible mine was simply defective.
I did every imaginable thing to try to get this mouse to work. The mouse I had was brand new from Razor, but it was running an old firmware. I did a firmware update to version 1.03.00 (it was running version 1.02.00 when it arrived). I can confirm that firmware update did NOT fix the problem with the phantom clicks.
I also set all the related devices in my device manager to not go to sleep, including the USB slots. That did NOT fix the problem with the phantom clicks.
I also, at the advice of chatGPT, did a bunch of registry edits, none of which fixed the problem.
I also, at the advice of chatGPT, wrote my own c# application, which attempted in various ways to force the mouse to release the phantom click, at least when the computer would wake up. I was trying to mimic the behavior of pressing CTRL+ALT+DEL. After many hours of coding, I was unable to programmatically correct the problems with this mouse.
I really wanted this mouse to work because it's wireless, and its small, so it fits my daughter's hand.
I'm now having to return this mouse at my time and expense, no option for replacement, lost whatever discount I got from the previous sale, and I lost days of my life trying to fix this mouse, and I lost some of my software and settings from the reinstallation of windows, so I have work to do just to get my daughter's computer back to normal again. Terrible experience.
Conclusion: Ask ChatGPT about this mouse and it'll tell you that other users have reported issues with this mouse not clicking properly. It will tell you that this is a known issue with this mouse.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 7, 2025
★★★★★ 5
Perfect mouse. Period.
Color: Black and Gold, Color: Black and Gold
LOVVVVEEEEE this mouse!!!! 20/10. I bought this for my new laptop and it was such a great value. I fell in love with how smooth and sleek it is, and how incredibly QUIET it is! This is the main reason I bought it, for the silence and the fact that it doesn’t click click click. So I immediately bought one for work since the one I have at work is so loud and clackety. Game changer. These are amazing. Love how they look, love how they feel in my hand using them, love how they work when using a computer, love how they fall asleep and save battery when not in use, love how they don’t make clicking noises or scrolling noises. Sooooooo good!!! Oh and love the price.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 9, 2026
★★★★★ 5
Very durable and pretty 😍
Color: Mint Green to Purple
The purple-mint gradient color is so pretty. It works well and feels nice in my hand. The buttons don't make a lot of noise, which is a bonus. Good for the price and haven't had any issues yet. You only need one battery and it lasts a long time. Installation is easy, just plug in the USB receiver, switch the button underneath to ON and you're good to go. No Bluetooth needed.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 22, 2026
★★★★★ 5
great mouse
Color: Black and Gold
really good mouse, the sound isn’t loud, i love the little scroller and ive used it daily for a few months now and the batteries life has lasted since then and i haven’t had to change them out which is great. it’s very lightweight and it will go into i think maybe a resting mode after not using it for a few minutes and you have to click to turn it back on which i do like. it fits very nicely in my hand and it’s overall a great mouse.
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Reviewed in the United States on May 5, 2026
★★★★★ 5
Quite clicks and easy manueverability. Works great.
Color: White and Gold
I was very pleased when I opened the box and seen that the actual mouse looks exactly like the pictures. It is lightweight and glides easily along my mouse pad. The colors are gorgeous and makes it look very chic! And it works great. It was easy to set up, just plugged in the USB receiver and it was up and running right away. The clicks make a muffled sound that is kind of soothing.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 3, 2026
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